烟草野火病菌对细菌杀星的敏感性测定及敏感基线建立  

Sensitivity Determination and Sensitivity Baseline Establishment of Pseudomonas syringae pv. tabaci to Streptomycin· Saikuzuo

作　　者：陈长卿[1] 隋原[1] 杨丽娜[1] 周洪波 张文中 高洁[1] 

机构地区：[1]吉林农业大学农学院,长春130118 [2]桦甸市农业综合执法大队,桦甸132400 [3]公主岭怀德农业站,公主岭136100

出　　处：《吉林农业大学学报》2011年第6期600-603,共4页Journal of Jilin Agricultural University

基　　金：吉林省科技发展计划项目(20090211)

摘　　要：从黑龙江省和吉林省烟草主要种植区获得烟草野火病菌（Pseudomonassyringaepv．tabaci）菌株61个，采用抑菌圈法对50％细菌杀星WP进行了敏感性测定。结果表明：不同地理来源的烟草野火病菌对细菌杀星敏感性有一定差异，EC50=1．070-1．517μg／mL，平均1．421t．,μg／mL；黑龙江省鸡西的菌株h28为最敏感菌株，其ECν50为1．070μg／mL，而黑龙江宁安菌株h40敏感性最低，其ECν50值为1．517μg／mL。同时确定了两省烟草野火病菌对细菌沙星的敏感基线为1．421μg／mL，可用于对烟草野火病菌对细菌杀星的抗药性监测；烟草野火病菌对细菌杀星未产生抗药性。The fungicide sensitivity to streptomycin“ saikuzuo of sixty one strains of Pseudomonas syringae pv. tabaci （Pst）from tabacco regions in Heilongjiang and Jilin provinces was determined using the method of inhibition zone. The results showed there were sensitivity differences among the strains, and ECsovalue of all strains was lower than 2 μg/mL, which ranged from 1.070 to 1.517μg/mL, and the average value was 1.421 μg/mL. Strains h28（ECs01.070 μg/mL） from Jixi, Heilongjiang was the most sensitive to the fungicide, but h40（ECs01.517/μg/mL） from Ning＇an, Heilongjiang was strain of the low- est sensitivity. The sensitivity baseline of Pst strains to streptomycin” saikuzuo was determined as 1.421 μg/mL, whieh could be used for monitoring resistance of Pst strains to the fungicide. There wasno resistance of Pst strains to the fungicide.

关 键 词：烟草野火病菌 细菌杀星 敏感性 敏感基线 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]