EDTA提高Microtox检测有机化合物毒性灵敏度的研究  

The Study of Improvement of Microtox Sensitivity to Toxic Organic Compounds by EDTA

作　　者：郝春慧[1] 石长华 郭寅生[1] 刘红艳[1] 黄正[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院教育部环境与健康重点实验室,湖北武汉430030 [2]湖北出入境检验检疫局

出　　处：《检验检疫学刊》2011年第5期42-45,48,共5页Journal of Inspection and Quarantine

基　　金：湖北省科技攻关项目(2006AA201C33)

摘　　要：[目的]改善明亮发光杆菌细胞通透性、提高Microtox对有机污染物生物毒性检测的灵敏度。[方法]以乙二胺四乙酸二钠(EDTA-Na2)处理明亮发光杆菌,用疏水荧光探针N-苯基-1-萘胺(NPN)检测明亮发光杆菌通透性的改变,应用发光细菌急性毒性检测技术(Microtox)检测有机化合物在通透性改变前后的50%发光抑制浓度(EC50)。[结果]加入EDTA后菌体对NPN的吸收系数明显增加,40mM的ED-TA可使明亮发光杆菌对NPN的吸收系数增加47.7%。未经EDTA处理时,1,10-二氮杂菲、1,4-对位苯醌、双酚A、4-异丙基苯酚经Microtox检测的EC50值分别为181.2、0.071、37.6、0.79mg/L,处理后分别降为115.1、0.054、26.24、0.603mg/L。[结论]EDTA可以提高明亮发光杆菌对有机化合物的通透性,使Microtox检测生物毒性的灵敏度提高。[Objective]To improve the permeability of Photobacterium phosphoreum and the sensitivity of Microtox to toxic organic compounds. [Methods]Photobacterium phosphoreum was treatd with EDTA, permeability of Photobacterium phosphoreum was detected by NPN and 50%luminescence inhibition （EC50） of organic compounds on Photobacterium phosphoreum was determinned by Microtox before and after the change of permeability. [Results]Absorption coefficient was improved significantly with addition of EDTA and 40mM EDTA produced 47.7% increase in NPN uptake by Photobacterium phosphoreum . The EC50 values of 1,10-phenanthroline, 1,4-Benzoquinone, bisphenol A and 4-Isopropylphenol were 181.2, 0.071, 37.6 and 0.79 mg/L respectively without EDTA treatment, while EC50 values decreased to be 115.1, 0.054, 26.24 and 0.603 mg/L correspondingly with the treatment of EDTA. [Conclusion]EDTA can improve the permeability of Photobacterium phosphoreum , and the sensitivity of Microtox test for biological toxicity.

关 键 词：发光细菌 通透性 MICROTOX EC50 

分 类 号：O621.2[理学—有机化学]