Integrin-linked kinase in gastric cancer cell attachment,invasion and tumor growth  

作　　者：Gang Zhao Li-Li Guo Jing-Yong Xu Hua Yang Mei-Xiong Huang Gang Xiao 

机构地区：[1]Department of General Surgery, Beijing Hospital, Peking University, 100730 Beijing, China [2]Department of Ophthalmology, People＇s Hospital, Peking University, 100044 Beijing, China

出　　处：《World Journal of Gastroenterology》2011年第30期3487-3496,共10页世界胃肠病学杂志（英文版）

基　　金：Supported by The grants from the Department of Anesthesiology and Intensive Care of Changhai Hospital,Shanghai,China

摘　　要：AIM： To investigate the effects of integrin-linked kinase （ILK） on gastric cancer cells both in vitro and in vivo. METHODS： ILK small interfering RNA （siRNA） was transfected into human gastric cancer BGC-823 cells and ILK expression was monitored by real-time quan- titative polymerase chain reaction, Western blotting analysis and immunocytochemistry. Cell attachment, proliferation, invasion, microfilament dynamics and the secretion of vascular endothelial growth factor （VEGF） were also measured. Gastric cancer cells treated with ILK siRNA were subcutaneously transplanted into nude mice and tumor growth was assessed. RESULTS： Both ILK mRNA and protein levels were significantly down-regulated by ILK siRNA in human gastric cancer cells. This significantly inhibited cell attachment, proliferation and invasion. The knockdown of ILK also disturbed F-actin assembly and reduced VEGF secretion in conditioned medium by 40% （P 〈 0.05）. Four weeks after injection of ILK siRNA-transfected gastric cancer cells into nude mice, tumor volume and weight were significantly reduced compared with that of tumors induced by cells treated with non-silencing siRNA or by untreated cells （P 〈 0.05）. CONCLUSION： Targeting ILK with siRNA suppresses the growth of gastric cancer cells both in v/tro and /n vivo. ILK plays an important role in gastric cancer progression.AIM:To investigate the effects of integrin-linked kinase(ILK)on gastric cancer cells both in vitro and in vivo.METHODS:ILK small interfering RNA(siRNA)was transfected into human gastric cancer BGC-823 cells and ILK expression was monitored by real-time quantitative polymerase chain reaction,Western blotting analysis and immunocytochemistry.Cell attachment,proliferation,invasion,microfilament dynamics and the secretion of vascular endothelial growth factor(VEGF) were also measured.Gastric cancer cells treated with ILK siRNA were subcutaneously transplanted into nude mice and tumor growth was assessed.RESULTS:Both ILK mRNA and protein levels were significantly down-regulated by ILK siRNA in human gastric cancer cells.This significantly inhibited cell attachment,proliferation and invasion.The knockdown ofILK also disturbed F-actin assembly and reduced VEGF secretion in conditioned medium by 40%(P<0.05).Four weeks after injection of ILK siRNA-transfected gastric cancer cells into nude mice,tumor volume and weight were significantly reduced compared with that of tumors induced by cells treated with non-silencing siRNA or by untreated cells(P<0.05).CONCLUSION:Targeting ILK with siRNA suppresses the growth of gastric cancer cells both in vitro and in vivo.ILK plays an important role in gastric cancer progression.

关 键 词：Gastric cancer Integrin-linked kinase Smallinterfering RNA Cell attachment Cell proliferation Cellinvasion Cell microfilament dynamics Vascular endo-thelial growth factor Nude mice 

分 类 号：Q255[生物学—细胞生物学] Q26