阿司匹林对人胶质母细胞瘤U87细胞系放射敏感性的影响  

Effect of aspirin on radiosensitivity of human glioblastoma multiforme cell line U87

作　　者：张兰兰[1] 彭苹[2] 吴炳义[1] 

机构地区：[1]南方医科大学南方医院临床医学实验研究中心,广州510515 [2]南方医科大学珠江医院特需医疗中心,广州510282

出　　处：《中华神经医学杂志》2011年第11期1110-1114,共5页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金海外青年学者合作研究基金（30628018）;广东省医学科学技术研究基金（A2008427）

摘　　要：目的观察阿司匹林（ASA）对人胶质母细胞瘤U87细胞系放射敏感性的影响并探讨其可能机制。方法常规培养U87细胞，CCK-8法检测16、8、4、2、1、0．5mmol／LASA对细胞抑制率和1minol／LASA预处理对2、4、8Gy6MV—X射线单次照射后细胞抑制率的影响，计算ASA对这3种剂量射线的放射增敏比；选取1mmol／LASA和8Gy照射剂量进行实验，分为对照组、照射组、ASA组、ASA＋照射组，流式细胞仪检测细胞凋亡和NF-κB的含量，激光共聚焦显微镜观察NF—κB的表达。结果与空白对照组比较，0．5、1mmol／LASA组细胞抑制率的差异无统计学意义（P〉0．05）；ASA对8Gy射线的放射增敏比高于2、4Gy，差异有统计学意义（P〈0．05）。流式细胞仪检测结果显示，与照射组比较，ASA＋照射组细胞凋亡率较高，NF-κB含量较低，差异均有统计学意义（P〈0．05）。激光共聚焦显微镜下观察显示，照射组细胞NF-κB在细胞质、细胞核均有表达，与ASA＋照射组细胞比较表达较强。结论ASA可提高胶质母细胞瘤的放射敏感性，它可能通过抑制NF-κB的表达而增加由射线介导的胶质瘤细胞的凋亡，从而增强细胞对射线的反应。Objective To observe the effect of aspirin （ASA） on radiosensitivity of human glioblastoma multiforme cell line U87, and explore its mechanism. Methods Routine culture of U87 cell line was performed; inhibitory effect of different doses of ASA （16, 8, 4, 2, 1 and 0.5 mmol/L） on the proliferation of U87 cells and the influence of 1 mmol/L ASA pretreatment on the proliferation of U87 cells after single fraction irradiation with 2, 4 and 8 Gy 6MV-X ray were detected with CCK-8; the radiation enhancement ratio （RER） of ASA on these 3 different radiation doses were calculated. We chose 1 mmol/L ASA and 8 Gy X-ray for further experiment; the U87 cells were divided into control group, radiation treatment group, ASA treatment group and radiation plus ASA treatment group; the changes of apoptosis rate and the level of NF-κB in each group were detected by flow cytometry （FCM）, and the expression of NF-κB was observed by laser scanning confocal microscope. Results As compared with that of control group （not given ASA）, the inhibitory effect on proliferation of U87 cells in 1 and 0.5 mmol/L ASA treatment groups was not significantly different （P〉0.05）. The RER in radiation dose of 2, 4 and 8 Gy induced by ASA was （0.155±0.008）, （0.205±0.017） and （0.392±0.024）, respectively; 8 Gy treatment group had a significantly higher RER than 2 and 4 Gy treatment groups （/）〈0.05）. FCM showed that ASA plus 8 Gy radiation treatment could increase the apoptosis rate of U87 from （7.74%±0.43%） to （12.58%±0.94%）, however, it could decrease the level of NF-κB in U87 cells from （96.65%±2.79%） to （77.06%±2.89%）; significant differences between control group and ASA plus radiation treatment group were noted （P〈0.05）. Laser scanning confocal microscope showed that the expression of NF-κB mainly appeared in the cytoplasm ad nucleus after irradiation, and its expression could be inhibited by ASA. Conclusion ASA could increase the radiosensitivity of U87 cells by inhibiting

关 键 词：胶质母细胞瘤 阿司匹林 放射敏感性 

分 类 号：R965[医药卫生—药理学]