食蟹猴胚胎干细胞向间充质前体细胞诱导分化及鉴定  

Induction and Identification of Mesenchymal Precursor Cells from Cynomolgus Monkey Embryonic Stem Cells

作　　者：任振华[1,2] 王佳茵[1] 朱宛宛[1] 邹春林[1] 张愚[1] 

机构地区：[1]首都医科大学宣武医院细胞生物室,北京100053 [2]安徽医科大学人体解剖学教研室,合肥230032

出　　处：《中国细胞生物学学报》2011年第11期1197-1203,共7页Chinese Journal of Cell Biology

基　　金：高等学校博士学科点专项科研基金(No.20091107120004);安徽医科大学博士科研资助基金(No.XJ201008)资助项目~~

摘　　要：间充质干细胞(mesenchymal stem cells,MSCs)可以诱导分化成脂肪、软骨、骨骼和骨骼肌细胞,并可作为骨骼、软骨或肌肉移植中的再生干细胞,广泛应用于细胞治疗和组织工程。胚胎干细胞(embryonic stem cells,ESCs)具有体外培养无限增殖和多向分化的特性,能被诱导分化为机体几乎所有的细胞类型。该研究通过无血清条件下诱导食蟹猴ESCs形成类胚体(embryoid bodies,EBs),然后在血清条件下贴壁分化EBs成间充质前体细胞(mesenchymal precursor cells,MPCs),再经过长期体外培养,纯化和扩增MPCs。结果显示,纯化后的MPCs具有MSCs生物学特征,并能在体外诱导分化成脂肪细胞和骨细胞。将这些细胞皮下注射给SCID小鼠,并未发现形成肿瘤,提示食蟹猴ESCs来源的MPCs具有一定的安全性。Mesenchymal stem cells （MSCs） have received considerable attention in recent years for various therapeutic approaches. The reason is that MSCs have the ability to expand in vitro and differentiate into osteogenic, adipogenic and chondrogenic lineages, which could then be used for cell therapy and tissue engineering. Embryonic stem cells （ESCs） are pluripotent cells with unlimited proliferation and differentiating into any cell type of the body. In this study, cynomolgus monkey ESCs were cultured in the absence of serum to form embryoid bodies （EBs）, and then EBs were transferred for adherent culture in the presence of serum. Mesenchymal precursor cells （MPCs） migrated from EBs were selectively isolated and expanded. The results showed that MPCs derived from ESCs had the biological characteristics of mesenchymal stem cells （MSCs）, and could be differentiated into osteogenic and adipogenic cells. NOD/SCID mice injected with MPCs did not show signs of tumor development. The results suggested that MPCs derived from cynomolgus monkey ESCs had certain the safety of its clinical application.

关 键 词：食蟹猴胚胎干细胞 间充质前体细胞 诱导 分化 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]