Cry1Ab基因转化甘蔗及转基因抗虫植株的获得  

Genetic Transformation of CryIAb Gene into Sugarcane

作　　者：冯翠莲[1] 沈林波[1] 赵婷婷[1] 王俊刚[1] 熊国如[1] 张树珍[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所甘蔗研究中心,海南海口571101

出　　处：《热带农业科学》2011年第9期21-26,共6页Chinese Journal of Tropical Agriculture

基　　金：国家自然科学基金青年科学基金项目(No.31101197);现代农业产业技术体系建设专项基金(No.nycytx-24);中央级公益性科研院所基本科研业务费(No.ITBBZD0724)

摘　　要：构建玉米UBI启动子驱动Bt Cry1Ab基因的高效植物表达载体pUBTB,通过农杆菌介导法导入甘蔗愈伤组织,经过除草剂PPT三次连续筛选,共获得67株抗性植株,经PCR扩增检测,得到22株阳性植株,并对其进行RT-PCR检测,证明外源基因已经成功整合到其中的15株甘蔗基因组中,且得到了有效的表达。The plant expression vector pUBTB harboring CrylAb gene drivered by UBI promoter was constructed and introduced into sugarcane by Agrobatorium-mediated transformation, and 67 transformed plantlets were survived after three times successive PPT selection. These plantlets were detected by PCR and 22 plantlets showed positive. Furthermore, seven PCR positive plantlets were also detected by RT-PCR. The results showed that the foreign gene had been integrated into sugarcane genome and expressed.

关 键 词：甘蔗 CrylAb基因 植物表达载体 遗传转化 

分 类 号：S566.1[农业科学—作物学]