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机构地区:[1]西南林业大学林学院/西南山地森林资源保育与利用省部共建教育部重点实验室,云南昆明650224
出 处:《热带农业科学》2011年第9期48-50,共3页Chinese Journal of Tropical Agriculture
基 金:西南林业大学重点基金项目(No.200906Z)
摘 要:以2%草酸溶液为提取溶剂,超声提取维生素C。在254 nm波长下,以0.05 mol/L磷酸二氢钾∶甲醇(90∶10)为流动相,采用HPLC法,检测出石屏、普洱、西双版纳和建水共4个产地树头菜嫩叶中维生素C含量分别为0.79、1.4、1.5和1.6 mg/g,其中建水产地的维生素C含量最高,石屏产地的含量最低。该方法准确可靠,可作为树头菜中维生素C含量的测定。The extraction of vitamin C was measured by ultrasonic extraction and HPLC method, which using 2% oxalic acid solution as extracting solvent, and using 0.05 mol/L KH2PO4 solution-methanol (90 : 10) as mobile phase and the detection wavelength was at 254 nm. Under these conditions, the vitamin C content from the tender leaves of Crateva uailocularis in the four different regions, including Shiping, Pu'er, Xishuangbanna and Jianshui, was 0.79 mg/g, 1.4 mg/g, 1.5 mg/g, 1.6 mg/g respectively. And vitamin C content in Crateva uailocularis is the highest in Jianshui, the lowest in Shiping. This method was accurate and good for the determination of vitamin C in Crateva uailocularis.
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