ABA预处理对蝴蝶兰类原球茎耐脱水性及生理基础的影响  

Effects of ABA Pretreatment on Dehydration Tolerance and It's Physiological Bases of Phalaenopsis PLB

作　　者：刘福平[1] 

机构地区：[1]福建省亚热带植物研究所福建省亚热带植物生理生化重点公共实验室,福建厦门361006

出　　处：《热带作物学报》2011年第9期1715-1719,共5页Chinese Journal of Tropical Crops

基　　金：福建省自然科学基金资助项目(No.2009J01079)

摘　　要：探讨ABA溶液预处理蝴蝶兰类原球茎(PLB)对耐脱水性及生理学基础的效应,本实验观测H2O和ABA溶液分别预处理PLB的脱水成活率及脱水前后的相关生理指标。结果表明,80μmol/L的ABA溶液预处理PLB,显著降低失水速度,极显著提高脱水后成活率。新鲜PLB、H2O或ABA溶液浸泡的PLB三者之间的干物质重量、相对电导率和成活率均无显著差异。ABA溶液预处理能极显著提高PLB可溶性蛋白、热稳定蛋白、可溶性糖和蔗糖含量,明显地降低还原糖含量,也能使PLB的SOD活性极显著提高,POD、CAT活性均极显著降低,但总抗氧化能力极显著提高。所以,ABA溶液短时间处理蝴蝶兰PLB并不能明显改变重量和造成损伤,预处理能提高脱水保护物质含量和总抗氧化能力水平,是增强PLB脱水耐性的基础。Dehydration survival rate of PLB pretreated with H20 and ABA solution respectively, as well as physiological indices of PLB before and after desiccation were. preliminarily studied to reveal the effects of ABA solution pretreatment on the dehydration tolerance and it＇s physiological bases of Phalaenopsis PLB （protocorm-like body）. The result showed that： PLB pretreated with 80 ~mol/L ABA had obviously lower dehydration rate and very significantly higher survival rate after desiccation. There were not significant difference in dry weight, relative conductivity and survival rate after desiccation between fresh PLB, PLB pretreated with H20 and ABA solution respectively. Pretreatment of PLB with ABA solution could very significantly increase the contents of soluble protein, heat-stable protein, soluble carbohydrate and sucrose, respectively. Also, ABA could very significantly increase SOD activity of PLB and very significantly decrease the activitives of POD and CAT, but increase very significantly the total antioxidative capability of PLB. It is suggested that pretreatment of Phalaenopsis PLB with ABA solution in a short time could not change weigh indices and damage on PLB, and ABA could increase the content of protective substances and increase the total antioxidative capability, and that was the bases of elevating dehydration tolerance of PLB.

关 键 词：蝴蝶兰类原球茎 ABA预处理 耐脱水性 生理学基础 

分 类 号：Q945.78[生物学—植物学] S682.31[农业科学—观赏园艺]