地衣芽孢杆菌CHB6高芽孢形成率发酵条件的研究  

The Fermentation Conditions for High Sporulation Rate of Bacillus licheniformis CHB6

作　　者：林陈强[1] 谢廼鸿[1,2] 邱宏端[2] 郑力[1] 陈济琛[1] 

机构地区：[1]福建省农业科学院土壤肥料研究所,福建福州350013 [2]福州大学生物科学与工程学院,福建福州350002

出　　处：《热带作物学报》2011年第9期1746-1749,共4页Chinese Journal of Tropical Crops

基　　金：福建省自然科学基金(No.2009J01096);福建省属公益类科研基本科研经费闽财教(No.20090584);福建省科技厅重点项目(No.2009S0132)

摘　　要：以CHB6的细胞生物量和芽孢率为指标,采用正交试验方法,研究了无机盐离子对地衣芽孢杆菌CHB6的细胞生长量及芽孢形成的影响,通过优化获得地衣芽孢杆菌CHB6的细胞生长量和芽孢形成率均较高的培养基配方。结果表明,Mn2+离子对CHB6的影响最显著,最佳培养基配比为:糖蜜1.5%、大豆蛋白胨0.5%、MnSO4.H2O 0.04%、MgSO4.7H2O 0.05%、CaCl2 0.1%、K2HPO4.3H2O 0.1%、KH2PO4 0.125%、NaCl0.5%。细胞生长量可达4.8×109 CFU/mL,芽孢形成率达到90.6%。The indices of cell biomass and sporulation rate were used to optimize the components of the culture medium for Bacillus licheniformis CHB6. The results showed that different inorganic ions affected the growth of the strain CHB6 variously, and the best medium was molasses 1.5%, soybean meal 0.5%, MnSOa-H20 0.04%, MgSO4·7H20 0.05%, CaC12 0.1%, K2HPO4·3H20 0.1%, KH2PO4 0.125% and NaC1 0.5%. The largest number of bacterial cells could reach more than 4.8x109 CFU/mL and the sporulation rate could reach 90.6%.

关 键 词：地衣芽孢杆菌CHB6 芽孢率 正交优化 

分 类 号：Q939.9[生物学—微生物学]