利用SSR标记分析水稻抗瘟材料的遗传多样性  

SSR analysis of genetic diversity of rice germplasms resistant to blast

作　　者：邱琳[1] 李俊刚 陈英[1] 涂升斌[1] 

机构地区：[1]中国科学院成都生物研究所,成都610041 [2]仁寿县石咀乡农业服务中心,四川眉山620575

出　　处：《东北农业大学学报》2011年第10期20-25,共6页Journal of Northeast Agricultural University

基　　金：中国科学院对外合作重点项目(GJHZ0955)

摘　　要：选用分布于水稻12条染色体上与抗稻瘟病基因紧密连锁的86对SSR引物,对30份稻瘟病抗性水稻材料进行遗传多样性分析。结果表明,19对引物在30份材料间表现出多态性。UPGMA聚类分析结果表明,供试材料可分为6类,反映出供试材料抗性遗传相似度低,遗传背景差异较大。聚类结果与材料的田间抗性表现基本一致,遗传亲缘关系基本吻合,说明利用SSR标记分析水稻品种资源遗传基础具有一定的可靠性。试验为供试材料遗传多样性的研究利用和抗瘟新品种的选育提供了理论依据。Total 86 SSR primers located at 12 chromosomes in rice, which were closely linked with blast resistance genes, were selected to analyze the genetic diversity of 30 blast resistant rice materials. The results indicated that 19 SSR markers performed polymorphism in the 30 materials. The results of cluster analysis by UPGMA method showed that 30 materials could be clustered into six groups which reflected that those tested materials had low similarity in resistance inheritance and large variation in genetic background. The field resistance performance of those materials was basically consistent with the results of cluster analysis and genetic relationship was basically coincided, which showed that it was reliable to use SSR markers to analyze genetic basis of rice. The experiment provided theoretical basis both for the use of genetic diversity of the 30 materials and for breeding of new blast resistant rice varieties.

关 键 词：水稻抗瘟材料 SSR分子标记 遗传多样性 聚类分析 

分 类 号：S511[农业科学—作物学]