Simple and Rapid Hollow Fiber Liquid Phase Microextraction Followed by High Performance Liquid Chromatography Method for Determination of Drug-protein Binding  

作　　者：XI Guo-chen HU Shuang BAI Xiao-hong 

机构地区：[1]Department of Pharmaceutical Analysis, School of Pharmaceutical Science, Shanxi Medical University, Taiyuan 030001, P. R. China

出　　处：《Chemical Research in Chinese Universities》2011年第5期764-768,共5页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.81041084);the Natural Science Foundation of Shanxi Province, China(No.2007011086);the Undergraduate Innovation Fund of Taiyuan City, China(No.08122034)

摘　　要：A method was established using hollow fiber-liquid phase microextraction（HF-LPME） followed by high performance liquid chromatography（HPLC） to determine the concentration of the free（unbound） drug in the solution of the drug and protein. Measurements of drug-protein binding ratios and free drug concentrations were then analyzed with the Klotz equation to determine the equilibrium binding constant and number of binding sites for drug-protein interaction. The optimized method allows one to perform the efficient extraction and separation of free drug from protein-bound drug, protein, and other interfering substances. This approach was used to characterize the binding of the anticholinergic drugs atropine sulfate and scopolamine hydrobromide to proteins in human plasma and bovine serum albumin（BSA）. The results demonstrate the utility of HF-LPME method for measuring free drug concentrations in protein-drug mixtures and determining the protein binding parameters of a pharmacologically important class of drugs.A method was established using hollow fiber-liquid phase microextraction（HF-LPME） followed by high performance liquid chromatography（HPLC） to determine the concentration of the free（unbound） drug in the solution of the drug and protein. Measurements of drug-protein binding ratios and free drug concentrations were then analyzed with the Klotz equation to determine the equilibrium binding constant and number of binding sites for drug-protein interaction. The optimized method allows one to perform the efficient extraction and separation of free drug from protein-bound drug, protein, and other interfering substances. This approach was used to characterize the binding of the anticholinergic drugs atropine sulfate and scopolamine hydrobromide to proteins in human plasma and bovine serum albumin（BSA）. The results demonstrate the utility of HF-LPME method for measuring free drug concentrations in protein-drug mixtures and determining the protein binding parameters of a pharmacologically important class of drugs.

关 键 词：Liquid phase microextraction Drug-protein binding ratio Binding parameter Anticholinergic drug 

分 类 号：TQ028.8[化学工程] O657.72[理学—分析化学]