NKA-9型大孔树脂纯化蟾皮总生物碱工艺  

Purification Technology of Total Alkaloids from Skin of Bufo bufo Gargarizans by NKA-9 Macroporous Resin

作　　者：王元清[1,2] 严建业[2] 罗堃[2] 王璐[2] 师白梅[2] 汪兰[2] 

机构地区：[1]中南林业科技大学生命科学与技术学院,长沙410004 [2]湖南中医药大学药学院,长沙410208

出　　处：《中国实验方剂学杂志》2011年第22期6-8,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中南林业科技大学青年科学研究基金项目(2009015B);湖南省教育厅科学研究项目(11C1321);湖南省高校科技创新团队项目(湘教通[2010]212号)

摘　　要：目的:建立NKA-9型大孔树脂纯化蟾皮总生物碱的工艺条件。方法:以蟾皮总生物碱含量(5-羟色胺计)作为考察指标,优化NKA-9型大孔树脂吸附与解析的工艺条件。结果:优选出的最佳纯化工艺为NKA-9型大孔树脂在25℃进行吸附,样品溶液pH 9,上样量为4 BV,吸附时间为8～10 h,先用6 BV水洗,然后用3 BV70%的乙醇洗脱。结论:该工艺可用于蟾皮总生物碱的纯化。Objective：To establish purification technology of total alkaloids from skin of Bufo bufo gargarizans by NKA-9 macroporous resin.Method：The content of total alkaloid（5-HT） from skin of B.bufo gargarizans was chosen as index,optimized condition of adsorption and desorption of NKA-9 macroporous resin and established purification technology condition of total alkaloids from skin of B.bufo gargarizans.Result：Optimum purification process conditions were：used NKA-9 macroporous resin,adsorption temperature was 25 ℃,loaded 4 times the amount of column volume with pH of sample solution was 9;adsorption time was 8-10 h,firstly eluted with 6 times water of column volume,then eluted with 3 times 70% ethanol of column volume.Conclusion：Optimum process could be used for purification of total alkaloids from skin of B.bufo gargarizans.

关 键 词：NKA-9型大孔树脂 蟾皮总生物碱 纯化工艺 

分 类 号：R283.6[医药卫生—中药学]