通关藤药材C21甾体皂苷类成分特征图谱研究  

Study on Specific Chromatogram of Tenacissoside in Marsdenia tenacissima

作　　者：李媛媛[1] 倪艳[2] 李先荣[2] 卢方晋[2] 石任兵[1] 

机构地区：[1]北京中医药大学,北京100102 [2]山西省中医药研究院方剂研究所,太原030012

出　　处：《中国实验方剂学杂志》2011年第22期43-46,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山西省青年科技研究基金(2009021040-2);山西省卫生厅科技攻关计划项目(200915)

摘　　要：目的:通过建立通关藤药材C21甾体皂苷类成分的特征图谱,为科学评价药材质量提供理论依据。方法:色谱条件:Sinochrom ODS-BP分析柱(4.6 mm×250 mm,5μm),柱温室温,检测波长223 nm,流动相乙腈-0.1%磷酸水溶液(32∶68～85∶15)梯度洗脱,流速1 mL.min-1。结果:通关藤药材指纹图谱选定了17个共有峰,其中2号峰为通关藤苷A,建立了通关藤药材HPLC指纹图谱共有模式,得到不同产地通关藤药材的相似度数据。结论:建立的指纹图谱重复性好,具有较广泛的应用范围,可用于通关藤药材的HPLC指纹图谱评价其质量。Objective： To develop the fingerprint of Marsdenia tenacissima by UV-HPLC.Method：The RP-HPLC was performed on a Sinochrom ODS-BP column（4.6 mm×250 mm,5 μm）.The mobile phase consisted of acetonitrile-0.1% H3PO4 with gradient elution.The fingerprint was detected at 223 nm.Result： The mutual mode of HPLC-UV specific chromatogram was set up.14 common peaks were observed on the fingerprints of M.tenacissima,and the second peak was tenacissoside A.Conclusion： This method is simple and fast,thus it may be used for the fingerprint of M.tenacissima.It may also qualitatively and quantitatively reflect the content variation of the main ingredients in crude drugs,which would be helpful to improve the quality control level.

关 键 词：通关藤 通关藤苷A 高效液相色谱法 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]