益髓生血颗粒质量标准研究  

Study on Quality Control Standard of Yisui Shenxue Granules

作　　者：沈蒙[1] 刘起华[2] 康玉玲[1] 徐静静[1] 韦灵玉[1] 王和东[1] 吴志奎[2] 张玉杰[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]中国中医科学院广安门医院,北京100053

出　　处：《中国实验方剂学杂志》2011年第22期47-50,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重大新药创制科技重大专项(2009ZX09301-005-2-7)

摘　　要：目的:建立益髓生血颗粒的质量标准。方法:采用TLC对处方中的山茱萸,制何首乌,灸黄芪,当归,补骨脂和砂仁进行定性鉴别;用HPLC测定益髓生血颗粒中马钱苷的含量。结果:薄层图谱斑点清晰,阴性对照无干扰;马钱苷在0.13～0.78μg呈良好的线性关系,平均回收率为101.3%,RSD为2.02%。结论:建立的方法简便,准确,重复性好,精密度高,可用于益髓生血颗粒的质量控制。Objective： To establish the method for quality control of Yisui Shenxue granules.Method： The herb medicines including medical dogwood,prepared fleeceflower root,milkvetch root,chinese angelica,malaytea scurfpea fruit and villous amomrum fruit were identified by TLC and the content of loganin in Yisui Shenxue granules was determined by RP-HPLC.Result： The herbs can be effectly identified by TLC.Loganin was linear in the range of 0.13-0.78 μg,and the average recovery was 101.3%（RSD 2.02%）.Conclusion： The method is simple and rapid which can be used for the quality control of Yisui Shenxue granules.

关 键 词：益髓生血颗粒 薄层鉴别 马钱苷 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]