桔梗的HPLC-ELSD指纹图谱研究  

Fingerprint Analysis of Platycodonis Radix by HPLC-ELSD

作　　者：李文庭[1] 祝明[2] 马临科[2] 程勇 

机构地区：[1]浙江中医药大学药学院,杭州310053 [2]浙江省食品药品检验所,杭州310004 [3]浙江天草生物制品有限公司,浙江安吉313300

出　　处：《中国实验方剂学杂志》2011年第22期50-53,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立桔梗高效液相色谱-蒸发光散射检测器(HPLC-ELSD)指纹图谱的分析方法,研究不同产地桔梗药材的质量。方法:采用HPLC-ELSD,Welch Materials XB-C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1%甲酸溶液,梯度洗脱,流速0.8 mL.min-1,柱温30℃,ELSD检测器漂移管温度50℃,氮气流速1.2 L.min-1。结果:建立了12批桔梗的HPLC-ELSD指纹图谱,共找到16个共有峰,并确定了桔梗皂苷E,桔梗皂苷D3和桔梗皂苷D。结论:该方法具有良好的重复性和稳定性,为有效控制桔梗药材的质量提供了依据。Objective： To establish a method of fingerprint analysis for Platycodonis Radix by high performance liquid chromatography-evaporative light-scattering detector（HPLC-ELSD）.Method： HPLC was performed on a Welch Materials XB-C18 analytical column（4.6 mm × 250 mm,5 μm） with gradient elution of acetonitrile-0.1% formic acid at the flow rate of 0.8 mL·min-1 with ELSD detector.The column temperature was maintained at 30 ℃,the temperature of drift tube was set at 50 ℃,and the gas flow（N2） was set at 1.2 L·min-1.Result： The HPLC-ELSD chromatographic fingerprint of chemical constituents was established from the 12 batches of Platycodonis Radix and it had 16 characteristic common peaks.Conclusion： The developed method is repeatable and stable,which is helpful to control the quality of Platycodonis Radix.

关 键 词：桔梗 指纹图谱 高效液相色谱-蒸发光检测法 桔梗皂苷 

分 类 号：R284.1[医药卫生—中药学]