广西产桑椹HPLC指纹图谱研究  

Study on Chromatography Fingerprints of Morus alba by HPLC

作　　者：耿旦[1] 马雯芳[2] 丘琴[2,3] 甄汉深[2] 韦良宏[1] 蒋凌芳[2] 

机构地区：[1]钦州市第一人民医院,广西钦州535000 [2]广西中医学院,南宁530001 [3]成都中医药大学,成都611137

出　　处：《中国实验方剂学杂志》2011年第22期54-56,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：广西卫生厅项目(Z2009238)

摘　　要：目的:采用HPLC建立广西产桑椹的指纹图谱。方法:采用Agilent Zobax C18(4.6 mm×250 mm,5μm)色谱柱;以乙腈(A)-0.1%磷酸(B)为流动相进行梯度洗脱,检测波长360 nm,流速1.0 mL.min-1,柱温30℃。结果:检测了广西不同来源10批桑椹药材,标定了10个共有峰,相似度较高,建立的桑椹指纹图谱稳定性、重复性好。结论:桑椹HPLC指纹图谱可用于桑椹药材的鉴别及质量控制。Objective： To establish the chromatography fingerprint of Morus alba L.by HPLC.Method：The samples are separated on Agilent Zobax C18（4.6 mm×250 mm,5 μm） with acetonitrile-0.1% phosphoric acid as the mobile phase,gradient eluent,at the flow rate of 1.0 mL·min-1 at 30 ℃,the UV detection is seted at 360 nm.Result： Ten samples of M.alba in different origin of Guangxi was detected and 10 peaks in the chromatogram are common,there is a high similarity,the reproducibility and stability is good.Conclusion： The HPLC fingerprint can be used for identification of M.alba as well as quality control.

关 键 词：桑椹 指纹图谱 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]