HPLC同时测定妇炎康片中芍药苷、丹酚酸B、小檗碱  

Simultaneous Determination of Paeoniflorin,Salvianolic Acid B and Berberine in Fuyankang Pills by HPLC

作　　者：黄鸣清[1] 谢友良[2] 王德杭[2] 蒋东旭[2] 江滨[2] 赖小平[2] 

机构地区：[1]福建中医药大学药学院,福州350108 [2]广州中医药大学新药开发研究中心,广州510405

出　　处：《中国实验方剂学杂志》2011年第22期68-71,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立同时测定妇炎康片中芍药苷、丹酚酸B及小檗碱的含量测定方法。方法:采用HPLC,PhenomenexLuna C18(4.6 mm×250 mm,5μm),以乙腈-0.05 mol.L-1磷酸二氢钾(含0.1%磷酸)为流动相梯度洗脱,流速1.0 mL.min-1;检测波长230,265,286 nm。结果:芍药苷的线性范围为0.077 08～0.770 8μg(r=0.999 7),平均回收率为98.87%;丹酚酸B的线性范围为0.1139～1.139μg(r=0.999 9),平均回收率为99.31%;小檗碱的线性范围为0.027 8～0.278μg(r=0.999 9),平均回收率为100.65%。不同厂家妇炎康片中芍药苷、丹酚酸B、小檗碱的含量差异较大。结论:该方法简便,专属性、重复性好,可作为该制剂中芍药苷、丹酚酸B及小檗碱的含量测定方法。Objective： To establish a method of simultaneous determining effective components in Fuyankang pills.Method： The content of paeoniflorin,salvianolic acid B and berberine were determined by HPLC.The analytes were separated using a system of acetonitrile-0.04 mol·L-1 potassium dihydrogen phosphate solution containing 0.1% phosphoric acid as the mobile phase,and a linear gradient elution program.The flow rate was 1.0 mL·min-1 and the detection wavelengths of paeoniflorin,salvianolic acid B and berberine were 230,286,265 nm,respectively.Result： The HPLC method was found to be sensitive and linear with the range of 0.077 08-0.770 8 μg for paeoniflorin（r=0.999 7）,0.113 9-1.139 μg for salvianolic acid B（r=0.999 9）,and 0.027 8-0.278 μg for berberine（r=0.999 9）,and the average recoveries for paeoniflorin,salvianolic acid B and berberine were 98.87%,99.31% and 100.65%,respectively.There was great difference in the content of paeoniflorin,salvianolic acid B and berberine in Fuyankang pills from different manufacturers.Conclusion： The method is simple,specific and reproducible for the determination of paeoniflorin,salvianolic acid B and berberine in Fuyankang pills by HPLC.

关 键 词：妇炎康片 芍药苷 丹酚酸B 盐酸小檗碱 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]