大蓟生品及炭品中多糖不同提取方法的比较  

Comparison of Extraction Methods of Polysaccharides from Raw and Processing Cirsium japonicum

作　　者：龚千锋[1] 余香[1] 钟凌云[1] 王晓崴[1] 易炳学[1] 陈泣[1] 李慧莲[1] 杨巧玲[1] 朱龙涛[1] 任建锋[1] 

机构地区：[1]江西中医学院,南昌330004

出　　处：《中国实验方剂学杂志》2011年第22期94-97,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81060343)

摘　　要：目的:通过对几种不同的提取多糖方法的比较研究,寻找提取大蓟生品及炭品多糖效果较好的方法。方法:分别采用水提醇沉法、醇提除杂法、超声辅助法、碱提取法对中药大蓟生品及炭品进行提取,用苯酚-硫酸比色法测定多糖含量。结果:生品多糖4种方法测定的结果分别为1.587%,1.908%,2.283%,0.112%;炭品多糖测定的结果分别为2.142%,0.425%,2.302%,2.343%。结论:大蓟生品的多糖提取方法是超声辅助法提取的多糖含量较高,差异显著。大蓟炭的多糖提取方法是醇提除杂法提取的多糖含量较高,差异显著。Objective： Different extraction methods of polysaccharides were compared in order to optimize a better method to extract polysaccharides from the raw and processing Cirsium japonicum.Method： Four methods were used to extract the raw and processing C.japonicum.The four methodswere as follows：① water extraction followed by alcohol precipitation;② removing impurity by ethanol extraction;③ultrasonic auxiliary extraction;④extraction with alkali solution.Polysaccharides were detected by phenol sulfuric acid colorimetry.Result： The polysaccharide content extracted by above four different methods in the raw C.japonicum was 1.587%,1.908%,2.283% and 0.112%,and in the processing C.japonicum was 2.142%,0.425%,2.302% and 2.343%,respectively.Conclusion： The optimum method for the raw C.japonicum is ultrasonic auxiliary method which had significant difference while the best method for the processing of C.japonicum is removing impurity by ethanol extraction method.

关 键 词：大蓟生品及炭品 多糖 提取方法 

分 类 号：R284.1[医药卫生—中药学]