当归补血汤表征参数与其益气补血功效的关系初探  

Preliminary Studies on Relationship between Physical and Chemical Parameters and Effect of Invigorating Qi and Enriching Blood of Danggui Buxue Decoction

作　　者：马家骅[1] 李霞[2] 熊永爱[2] 李楠[2] 杨明[2,3] 

机构地区：[1]西南科技大学,四川绵阳621010 [2]成都中医药大学,成都611137 [3]江西中医学院现代中药制剂教育部重点实验室,南昌330004

出　　处：《中国实验方剂学杂志》2011年第22期111-116,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30801550)

摘　　要：目的:研究当归补血汤理化表征参数与其益气补血功效的关系,探索利用理化参数表征中药汤剂质量的可行性。方法:采用4种提取工艺(8倍量水提取1次,每次1 h;8倍量水提取3次,每次2 h;10倍量水提取3次,每次1.5 h;12倍量水提取3次,每次1 h)制备当归补血汤,测定其25℃时的表面张力,pH,电导率和渗透压;将77只小鼠随机分为正常组、模型组、阳性组和当归补血汤1,2,3,4号组,每组11只,给药8 d(10 g.kg-1.d-1),第5天开始ip环磷酰胺40 mg.kg-1.d-1,连续3 d,第8天ip环磷酰胺80 mg.kg-1,末次给药1.5 h后测定小鼠体温和外周血象;将55只小鼠随机分为正常组、模型组和当归补血汤高中低剂量组,研究高中低剂量(20,10,5 g.kg-1.d-1)对环磷酰胺所致血虚小鼠的体温和外周血象的影响;最后,关联理化参数、药效和主要活性组分含量,并对试验数据进行统计和回归分析。结果:经过多项式回归分析,理化参数值与组分含量和药效的综合评分之和、药效综合评分的相关系数均大于0.920 0,说明当归补血汤的理化表征参数、组分含量和益气补血药效3者的相关性显著。结论:当归补血汤理化参数与其益气补血功效相关性强,初步表明可利用上述特征参数来表征当归补血汤体系的内在质量。Objective：To study relationship between physical and chemical parameters and effect of invigorating Qi and enriching blood of Danggui Buxue decoction,and to explore the feasibility of characterizing the quality of decoction by physical and chemical parameters.Method：Danggui Buxue decoction were prepared by four methods（extracting once with 8 times of water for 1 h;extracting three times with 8 times of water for 2 h;extracting three times with 10 times of water for 1.5 h;extracting three times with 12 times of water for 1 h）,of which physical and chemical parameters such as surface tension,pH value,conductivity and osmotic pressure were determined at 25 ℃.Seventy-seven mice were randomly divided into 7 groups,with 11 in each group.Danggui Buxue decoction groups,E Jiao compound group,Blank group and model group were treated by Danggui Buxue decoction,E Jiao compound,normal saline respectively for eight days.Blood deficiency model mice were made by injecting cyclophosphamide from 5th to 8th day.Temperature and peripheral hemogram of mice were determined at 1.5 h after the last administration.Fifty-five mice were randomly divided into blank group,model group,high-dose of Danggui Buxue decoction group,moderate-dose of Danggui Buxue decoction group and low-dose of Danggui Buxuedecoction group,with 11 in each group,whose effects on temperature and peripheral hemogram of blood deficiency model mice induced by injecting cyclophosphamide were studied too.Finally,relationships among physical and chemical parameters,effect and the content of main active ingredients of Danggui Buxue decoction were studied,and mathematical analysis and regression analysis were used to analyze these data.Result： Through polynomial regression analysis,not only correlation coefficient between physical and chemical parameters and the score of component content and effect,but also correlation coefficient between physical and chemical parameters and the score of effect were more than 0.920 0.It showed that physical and chemical paramete

关 键 词：当归补血汤 理化参数 益气补血 主要活性组分 

分 类 号：R285.5[医药卫生—中药学]