藏党参皂苷对睾丸间质细胞的影响及作用机制  

Effect and Mechanism of Codonpsis thalictrifolia Saponin on Leydig Cells

作　　者：郑娟[1] 赵莹[1] 王丽蕃[1] 胡华刚[1] 徐斯凡[1] 

机构地区：[1]中央民族大学中国少数民族传统医学研究院,北京100081

出　　处：《中国实验方剂学杂志》2011年第22期145-148,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30973956)

摘　　要：目的:本研究以藏党参的正品长花党参Codonpsis thalictrifolia Wall.为研究对象,对藏党参皂苷对睾丸间质细胞功能的影响进行了初步研究。方法:利用不同质量浓度的藏党参皂苷对大鼠睾丸间质细胞进行体外培养,并测定细胞分泌睾酮的含量,cAMP的含量以及MTT吸光度。结果:藏党参皂苷质量浓度为0.4 g.L-1时,可以显著升高体外培养的大鼠睾丸间质细胞分泌睾酮的含量(P<0.05),提高cAMP的含量(P<0.01),并降低MTT吸光度(P<0.01)。结论:藏党参总皂苷可使单个睾丸间质细胞的分泌能力增强,可能由PKA通路介导。Objective： To study effects of Codonpsis thalictrifolia on leydig cells.Method： Leydig cells were collected and cultured in vitro with different concentration of saponin to test quantity of testerone and cAMP and MTT absorbance.Result： When saponin concentration was 0.4 g·L-1,quantity of testerone was significantly increased（P0.05）,content of cAMP was significantly increased（P0.01）,and MTT absorbance was significantly reduced（P0.01）.Conclusion：The saponin of C.thalictrifolia can strengthen the capability of single leydig cell to secrete testerone,which is perhaps induced by the pathway of PKA.

关 键 词：藏党参 皂苷 睾丸间质细胞 

分 类 号：R285.5[医药卫生—中药学]