大鼠腹腔肥大细胞与RBL-2H3细胞脱颗粒方法的建立及其在中药注射剂安全性评价上的应用  

Studies on Degranuation of Rat Peritoneal Mast Cells and RBL-2H3 Cells Caused by Traditional Chinese Medicine Injections

作　　者：李咏梅[1] 刘炯[1] 赵源[1] 汤家铭[1] 

机构地区：[1]上海中医药大学实验动物中心,上海201203

出　　处：《中国实验方剂学杂志》2011年第22期152-157,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：科技部重大新药创制基金项目(2009ZX09502-002)

摘　　要：目的:建立大鼠体外肥大细胞以及RBL-2H3细胞脱颗粒试验的方法,评估2种细胞脱颗粒方法对中药注射剂(traditional Chinese medicine injections,TCMI)引起脱颗粒的可行性。方法:提取大鼠腹腔肥大细胞(rat peritoneal mast cell,RPMC),优化实验条件,用阳性药物C48/80和各种TCMI等与其共培养,通过甲苯胺蓝染色法计数腹腔MC脱颗粒率;通过RBL-2H3细胞与TCMI共同培养,底物显色法检测β-氨基己糖苷酶释放率。结果:阳性对照品C48/80的浓度与RPMC脱颗粒有一定的量效关系;参麦、冠心宁、痰热清、清开灵和生脉5种TCMI能显著引起细胞脱颗粒,并与药物浓度有一定的正相关性;RBL-2H3细胞脱颗粒方法中参麦、黄芪、银杏、痰热清、丹香冠心、清开灵和生脉7种TCMI能是β-氨基己糖苷酶释放率升高。结论:一些TCMI能引起体外RPMC和RBL-2H3细胞脱颗粒,2种细胞脱颗粒方法在评价一些TCMI可能引起类过敏反应方面具有一定的互补性与可行性。Objective： Evaluating the feasibility of the degranulation methods in rats mast cells in vitro and in RBL-2H3 cells in vitro caused by traditional Chinese medicine injections（TCMI）.Method： Mast cells were gathered from rats peritoneal,the experimental conditions were optimized,the positive drug C48/80 and various TCMI were incubated to observe rat peritoneal mast cells degranulation caused by TCMI,through counting the degranulation rate of rat peritoneal mast cells whitch staining by toluidine blue.The positive drug C48/80 and various TCMI with RBL-2H3 cells were incubated to,detect the release rate of β-hexosaminidase by the method of substrate＇s colouration.Result： It showed certain concentration-response relations between the concentration of positive drug C48/80 and the degranulation rate of rat peritoneal mast cells;Shenmai,Guanxinning,Tanreqing,Qingkailing and Shengmai,total significantly caused mast cell degranulation,while the degranulation of mast cell and the concentration of drug had a certain positive correlation;and Shenmai,Huangqi,Yinxing,Tanreqing,Danxiang,Guanxin,Qingkailing,Shengmai which the release rate of β-hexosaminidase highen by RBL-2H3 cells.Conclusion： Some TCMI can cause rat peritoneal mast cells and RBL-2H3 cells degranulation in vitro,so they have certain potential application values and complementarity with the two methods evaluting anaphylactoid reaction caused by traditional Chinese medicine injection.

关 键 词：类过敏反应 中药注射剂 肥大细胞脱颗粒 RBL-2H3细胞 β-氨基己糖苷酶释放率 

分 类 号：R283.6[医药卫生—中药学]