清络通痹颗粒含药血清对破骨细胞骨吸收的影响  

Effects of Qingluo Tongbi Granule-containing Serum on Bone Resorption by Osteoclasts

作　　者：周玲玲[1] 梁晓雯[1] 柳璋璞[1] 方泰惠[1] 周学平[1] 

机构地区：[1]南京中医药大学,江苏省中药药效与安全性评价重点实验室,南京210029

出　　处：《中国实验方剂学杂志》2011年第22期168-172,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省高校自然科学基金项目(08KJB360006);教育部留学回国人员科研基金项目(2006331)

摘　　要：目的:确定清络通痹颗粒(QLT)含药血清的制备方法并观察其对体外培养破骨细胞的活性和骨吸收的影响。方法:选用高效液相色谱外标一点法测定,QLT含药血清中青藤碱的含量以确定含药血清的制备条件;乳鼠破骨细胞1×108/mL加入96孔板,将QLT 3.6,7.2,14.4 g.kg-1给药的大鼠含药血清1∶10稀释,分别加入各孔培养48 h,观察其对破骨细胞增殖、抗酒石酸酸性磷酸酶(TRAP)活性及破骨细胞形成的骨吸收陷窝的影响。结果:QLT 7.2,14.4 g.kg-1给药的含药血清可明显抑制破骨细胞的增殖能力和吸收陷窝面积(P<0.05,P<0.01),QLT 3.6,7.2,14.4 g.kg-1给药的含药血清可明显抑制破骨细胞的TRAP活性(P<0.05,P<0.01)。结论:QLT含药血清可抑制破骨细胞增殖、TRAP活性和骨吸收能力,对破骨细胞引起的骨质破坏产生抑制作用。Objective： To observe effects of Qingluo Tongbi granule-containing serum on bone resorption of osteoclasts in vitro.Method： The content of sinomenine in Qingluo Tongbi granule-containing serum was detected by HPLC in different time point to decide the method for obtaining the drug-containing serum.The osteoclasts from newborn rats were cultured and the proliferation,TRAP activity and bone resorption were detected.Result： The rats were given the drugs for 7 days and the drug-containing serum was obtained one hour after the last administration.The serum could inhibit the proliferation,TRAP activity and bone resorption area of osteoclasts.Conclusion： Qingluo Tongbi granule-containing serum could restore the bone erosion by inhibiting proliferation,TRAP activity and bone resorption ability.

关 键 词：清络通痹颗粒 含药血清 破骨细胞 骨吸收 

分 类 号：R285.5[医药卫生—中药学]