献血者血小板血型1～16抗原基因多态性分析及HPA血型库的建立  

Analysis of 1-16 HPA Antigen Gene Polymorphism to Blood Platelet Donors and Blood Type Library Building

作　　者：陈永兰[1] 杨俊鹏[1] 

机构地区：[1]天水市中心血站,甘肃天水741000

出　　处：《西部中医药》2011年第11期58-60,共3页Western Journal of Traditional Chinese Medicine

摘　　要：目的:分析天水地区献血者血小板血型1～16抗原基因多态性,找出有临床意义的血小板抗原,建立本地区HPA血型库。方法:采用SSP-PCR法对天水地区172名血小板献血者进行HPA-1～16抗原32个等位基因检测分析,并与不同人群进行比较分析。结果:已检的样本中,通过Hardy-Weiberg吻合度检验,P>0.05,即期望的基因频率和检测到的基因频率是吻合的。其中以HPA-2、3、15最为复杂,各种基因型(a/a、a/b、b/b)均能见到;HPA-5、6的杂合子基因型较少见,分别为2例和4例;HPA-4、7、8、9、10、11、12、13、14、16均表现为单倍体型。结论:天水地区人群中,HPA-2、3、15最为复杂,抗原暴露率高,较易产生抗体,可为临床血小板输注提供免疫学依据。同时据此建立了天水地区人群的HPA血型库。Objective：To analyze 1-16 HPA antigen gene polymorphisms for blood platelet donors,identify platelet antigen with clinical significance,and establish HPA blood type library in the Tianshui area.Method：By SSP-PCR method in the Tianshui area,32 alleles of 172 platelet donors for HPA-1 -16 antigen detection were analyzed and compared with different groups of people.Result： In the samples seized,the expected gene frequencies and gene frequencies detected are consistent by fit test of Hardy-Weiberg goodness （P〉0.05）.The HPA-2,3,15 is most complex, a variety of genotype （a/a,a/b,b/b） can been seen, but heterozygous genotypes of HPA-5,6 are rare,respectively 2 cases and 4 cases. HPA-4,7,8,9,10,11,12,13,14,16 showed haplotypes.Conclusion：In Tianshui area populations,HPA-2,3,15 is most complex because of high antigen exposure that easily produces antibodies,which can provide clinical immunological basis for platelet transfusion. Meanwhile HPA blood librariy was established in Tianshui area.

关 键 词：血小板血型 基因多态性 HPA血型库 

分 类 号：R457.1[医药卫生—治疗学]