白细胞滤除对不同时间制备的血浆质量的影响  

Effect of leukocyte depletion on the quality of plasma frozen within 24 hours of collection

作　　者：夏代全 黄扑 王世春[3] 王泽荣[3] 刘凤君[3] 黎儒青[3] 郭辉[4] 赵树铭[3] 

机构地区：[1]重庆市血液中心,重庆400015 [2]重庆市黔江中心血站 [3]第三军医大学西南医院输血科中国人民解放军重庆血站 [4]第三军医大学西南医院检验科

出　　处：《中国输血杂志》2011年第10期839-841,共3页Chinese Journal of Blood Transfusion

摘　　要：目的评价白细胞滤除对不同时间制备的血浆质量的影响。方法将24例400ml全血(4℃)采集6h内用无菌接驳机一分为二,1份6h内过滤去白再制备血浆(对照组,新鲜血浆,FP),另1份置4℃过夜(18～24h)后过滤去白再制备血浆(实验组,24h血浆,P24)。2组均于滤前滤后留取血浆标本,并立即置-30℃保存。3d后于37℃解冻后进行体外质量评估:观察外观、检测主要不稳定凝血因子(FⅤ、FⅦ、FⅧ)及Fib,以及主要生化指标含量。结果过滤处理对FP和P24均无明显影响;与滤后FP比较,滤后P24的FⅧ含量显著偏低,其他凝血因子含量差异不具统计学意义,生化指标中K+、LDH及FHb差异具统计学意义,其他指标差异不具统计学意义。结论除FⅧ外,白细胞滤除对FP24质量无显著影响。Objective To investigate the quality of plasma prepared from leukodepleted whole blood（WB） .Methods WB was collected according to standard procedures from normal volunteer donors（n=24） ,and then was divided into two equal units no later than 6 hours after collection.One unit was immediately leukodepleted and plasma separated（control group） ,and the other unit was stored overnight at 4℃ and then leukodepleted（experimental group） and plasma separated.All plasma was held in-30℃ refrigerator before testing.Plasma coagulation factors,FⅤ,FⅦ,FⅧ,and Fib and biochemical indicators were tested.Results But,in comparison to control group,experimental group plasma exhibited a lower FⅧ,but other coagulation factors were not significantly reduced.There were also significant statistical differences between their K＋,LDH,FHb,but plasma of both groups met current quality standards.Conclusion Leukocyte depletion does not affect the quality of plasma frozen within 24 h of collection（FP24）.

关 键 词：24h冰冻血浆 白细胞滤除 质量研究 

分 类 号：R457.14[医药卫生—治疗学]