MAPK信号转导在实验性重症急性胰腺炎大鼠中的作用  

The Role of MAPK Signal Transduction in Rats with Experimental Severe Acute Pancreatitis

作　　者：陈旭丹 王坤[2] 沈耀[2] 王平江[2] 党胜春[2] 

机构地区：[1]江苏省句容市白兔镇行香中心卫生院,江苏句容212402 [2]江苏大学附属医院普外科,江苏镇江212001

出　　处：《临床医学工程》2011年第11期1701-1702,共2页Clinical Medicine & Engineering

基　　金：江苏大学临床医学科技发展基金项目(编号:JLY20080018)

摘　　要：目的探讨p38MAPK信号转导通路在SAP中的作用及其特异性抑制剂SB203580对胰腺的保护作用。方法 SD大鼠48只,随机分为对照组(C)、胰腺炎组(P)及SB203580干预组(T)。各组于制模型后2及6小时分为2组,每组8只,用于抽取血样及胰腺组织学观察。P组采用胰腺被膜下均匀注射法制作模型,开腹后自胰尾至胰头方向被膜下均匀注射5%牛磺胆酸钠(4ml/kg),C组进行同样操作,仅胰腺被膜下注射等量生理盐水,T组术前灌胃给予SB203580(10mg/kg)。结果胰腺评分见C组胰腺病理正常,T组胰腺病理损害程度较P组明显减轻(P<0.05)。SAP大鼠模型诱导成功后,P组各时间点血清TNF-α浓度较C组明显升高(P<0.01),T组血清TNF-α浓度较P组比较明显下降(P<0.01)。P组各时间点胰腺p38MAPK表达较C组明显,T组各时间点胰腺p38MAPK表达较P组下降。结论 p38MAPK信号转导通路在SAP的发展中起着重要作用,其特异性抑制剂SB203580对胰腺的保护作用。Objective To investigate the role of the p38MAPK signaling pathway in severe acute pancreatitis （SAP） and its specific inhibitor SB203580 on the protective effect of the pancreas. Methods Forty-eight SD rats were randomly divided into control group （C）, severe acute pancreatitis （P） and SB203580 group （T）. For each group, it was further divided into 2 h and 6 h subgroup, respectively. All the rats were anesthetized with an intraperitoneal injection of 25% urethane （1.5 g/kg）. SAP models were induced by injection of 50 g/L sodium taurocholate （4 mL/kg） under the pancreatic membrane. In the normal control group, the rats received isovolumetric injection of 9 g/L normal saline solution. Preoperative treatment group was given orally SB203580 （10 mg/kg） in the T group. The blood samples of the rats in each group were obtained via superior mesenteric vein measuring levels of TNF-α. Results Pancreatic pathological scores were higher in P group than in C group throughout the experimental course （P 0.01）. Histopathologic scores in T group were lower than that of P group at 2 h and 6 h. Levels of serum TNF-α in P group were significantly higher than those of C group, respectively （P 0.01） . Levels of serum TNF-α were significantly decreased significantly in T group than those of P group at 2 h and 6 h （P 0.01）. At each time point, p38MAPK expression of pancreas in P group was significantly higher than group C and significantly decreased in T group than that in P group. Conclusion p38MAPK signal transduction pathway plays an important role in the development of SAP and its specific inhibitor SB203580 has the protective effect on the pancreas.

关 键 词：重症急性胰腺炎 丝裂原活化蛋白激酶 大鼠 

分 类 号：R657.51[医药卫生—外科学]