河北承德地区两个温泉中细菌的多样性分析  

Bacterial diversity analysis of two hot springs in Chengde,Hebei

作　　者：张丽娜[1] 郝春博[1] 李思远[1] 周训[1] 冯传平[1] 

机构地区：[1]中国地质大学(北京)水资源与环境学院,北京100083

出　　处：《微生物学通报》2011年第11期1618-1625,共8页Microbiology China

基　　金：国家自然科学基金项目(No.40802059;40972163);中央高校基本科研业务费专项资金资助项目(No.2010ZD03;2011YXL035)

摘　　要：通过构建16S rDNA克隆文库,对承德地区两温泉中的细菌多样性水平及系统发育关系进行了初步研究。研究表明:68°C的A11文库中阳性克隆的16S rDNA序列分属5个细菌类群,分别为Firmicutes(6.25%)、Deinococcus-Thermus(25.0%)、Gammaproteobacteria(12.5%)、Betaproteobacteria(50.0%)、Alphaproteobacteria(6.25%);而74.5°C的A12文库仅属于一个细菌类群:厚壁菌门(Firmicutes)。两温泉中细菌多样性的差异表明,温度是影响温泉中细菌多样性水平的重要因素。此外,A11文库中克隆的16S rDNA序列与许多已知的可产色素的好氧菌相似性很高,而A12文库中的细菌多数为专性厌氧或兼性厌氧型,其中厌氧芽孢杆菌属(Anoxybacillus)中的Anoxybacillus flavithermus可以作为研究泉华形成的理想材料。The bacterial diversity and phylogenetic analysis of two hot springs in Chengde were inves-tigated and analyzed by construction of 16S rRNA gene clone libraries.The results showed that bacteria in sample A11（68 °C） could be divided into 5 groups,which were as follows： Firmicutes（6.25%）,Deinococcus-Thermus（25.0%）,Gammaproteobacteria（12.5%）,Betaproteo-bacteria（50.0%）,Al-phaproteobacteria（6.25%）.But sample A12（74.5 °C） contains only one group,Firmicutes.The differ-ence between the two samples revealed that the temperature was an important factor to affect the level of bacterial diversity in hot springs.Furthermore,a lot of 16S rDNA sequences in A11 clone library had high similarity to the aerobic bacteria that can produce pigment,while most bacteria in A12 clone li-brary belonged to obligate or facultative anaerobe,in which,Anoxybacillus flavithermus can be used as ideal material to research the formation of sinter.

关 键 词：温泉 16S RDNA 克隆文库 细菌多样性 

分 类 号：Q938.8[生物学—微生物学]