基于第二代测序技术的细菌基因组与转录组研究策略简介  

The brief introduction of research strategies for bacterial genome and transcriptome based on the next-generation sequencing technologies

作　　者：刘万飞[1,2] 王西亮[1,2] 赵宇慧[1,2] 曾瀞瑶 耿佳宁[1] 胡松年[1] 

机构地区：[1]中国科学院北京基因组研究所基因组科学及信息重点实验室,北京100029 [2]中国科学院研究生院,北京100049

出　　处：《微生物学通报》2011年第11期1705-1714,共10页Microbiology China

基　　金：中国科学院知识创新工程项目(No.KSCX2-EW-R-01-04)

摘　　要：随着基于第二代测序技术的细菌基因组与转录组研究越来越广泛,选择合适的研究策略变得越来越重要。就基于第二代测序技术的细菌基因组和转录组研究策略进行综述,并简要介绍细菌基因组和转录组研究中的机遇和挑战。综述细菌基因组与转录组研究的常规方法及步骤,并简要地介绍存在的问题。细菌基因组和转录组研究策略为大多数细菌的研究提供了一个相对完整的研究路线,同时也会促进其它领域的研究,如生命形成、生物进化、基础代谢、疾病、药物等。Recently,many bacterial genome and transcriptome studies have been performed based on the next-generation sequencing technologies.Therefore,how to select the appropriate research strate-gies is becoming increasingly important.In this paper,we discussed the research strategies of bacterial genome and transcriptome based on the next-generation sequencing technologies,and stated the opportunities and challenges in these fields briefly.We reviewed the conventional methods and procedures and presented the existing problems briefly for bacterial genome and transcriptome studies.The research strategies of bacterial genome and transcriptome provide a relatively complete pipeline for the majority of bacteria.Moreover,it will promote the research of other fields,such as the course of life,biological evolution,basal metabolism,disease and drugs.

关 键 词：细菌 基因组 转录组 第二代测序技术 

分 类 号：Q933[生物学—微生物学]