高残留农药福美双对斑马鱼胚胎脊索的特异性影响  

Specific Effects of the Highly-Persistent Pesticide Thiuram on Notochord Development in Zebrafish Embryos

作　　者：于永利[1,2] 杨景峰[1] 巴雅斯胡[1] 王思珍[2] 赵宝全[3] 董武[1] 

机构地区：[1]内蒙古自治区毒物与疾病监控重点实验室,内蒙古民族大学动物科技学院,内蒙古通辽028042 [2]内蒙古民族大学生命科学学院,内蒙古通辽028042 [3]军事医学科学院毒物药物研究所,北京100850

出　　处：《环境科学研究》2011年第11期1297-1304,共8页Research of Environmental Sciences

基　　金：国家自然科学基金项目(30360090);教育部新世纪优秀人才支持计划项目(NCET-04-0260);教育部回国留学人员支持项目

摘　　要：福美双在我国是一种广泛使用的二硫代甲氨基甲酸盐类农药,它的高残留性已经引起世界各国的重视.以斑马鱼胚胎作为试验动物模型,从分子水平探讨了福美双对胚胎脊索弯曲的毒性作用机制.结果表明,福美双造成斑马鱼胚胎脊索弯曲与重叠等形态学变化,脊索弯曲程度与福美双的染毒浓度呈正相关.通过Ⅱ型胶原蛋白抗体染色表明,福美双引起软骨发育的变化与Ⅱ型胶原蛋白表达的降低密切相关.试验进一步表明,斑马鱼胚胎适合作为农药毒性评价的模型.Thiuram is a widely used dithiocarbamate pesticide in China.Its high persistence has already gained attention in countries around the world.In this experiment,we used zebrafish（Daniorerio） embryos as an experimental animal model to study the mechanism of Thiuram toxicity to embryonic notochord bending at the molecular level.The results showed that thiuram exposure caused morphological changes in zebrafish embryos,including wavy notochord and notochord overlap.The intensity of wavy notochord was increased by thiuram exposure in a concentration-dependent manner.In addition,typeⅡ collagen antibody stains indicated that thiuram decreased the expression of typeⅡ collagen,which was associated with the development of the cartilage.This study further indicated that zebrafish embryos are effective models for assessing the toxicity of pesticides.

关 键 词：福美双 斑马鱼胚胎 毒性 脊索 

分 类 号：R994.6[医药卫生—毒理学]