人角质细胞生长因子-1转化番茄的研究  

Study on Tomato Transformation with Human Keratinocyte Growth Factor-1

作　　者：邹金美[1] 张辉煌[2] 张国广[1,2] 陈亮[2] 

机构地区：[1]漳州师范学院生物系,福建漳州363000 [2]厦门大学生命科学学院,福建厦门361005

出　　处：《云南民族大学学报（自然科学版）》2011年第6期454-457,共4页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：福建省自然科学基金(2010J01240)

摘　　要：人角质细胞生长因子在组织的损伤修复中起着重要的作用.以番茄子叶为外植体,通过根癌农杆菌介导法,将人KGF-1导入番茄中,共获得12株独立的抗性转化植株,PCR和DNA斑点杂交结果表明:KGF-1基因整合入番茄基因组中,为获得植物源表达的KGF-1蛋白打下了基础.Keratinocyte growth factor plays an important role in the tissue damage repair. Using sterile tomato cotyledons as explants, the human KGF - 1 gene had been introduced into the tomato genome by Agrobacterium - medi- ated transformation method. As a result, a total of 12 independent putative transgenic tomato plantlets were ob- tained. The results of PCR and DNA dot - blot hybridization showed that KGF - 1 gene was integrated into the tomato genome. This work has laid a foundation for further studies on the development of KGF - 1 proteins expressed in tomatoes.

关 键 词：角质细胞生长因子-1 番茄 遗传转化 

分 类 号：S641.2[农业科学—蔬菜学]