上海市静安区儿童腹泻中分离的宋内志贺菌脉冲场凝胶电泳分析  

INVESTIGATION ON THE PFGE MAP OF SHIGELLA SONNEI ISOLATED FROM DIARRHEA IN CHILDREN IN JINGAN DISTRICT OF SHANGHAI

作　　者：李颐[1] 崔生辉[2] 罗蓓[1] 胡雪明[1] 

机构地区：[1]上海市静安区疾病预防控制中心,上海200041 [2]中国药品生物制品检定所

出　　处：《现代预防医学》2011年第22期4700-4702,共3页Modern Preventive Medicine

摘　　要：[目的]研究上海市静安区2006～2008年儿童腹泻标本中分离的20株宋内志贺菌的耐药性和脉冲场凝胶电泳图谱特征。[方法]采用K-B法检测对16种抗生素的敏感性,并用WHONET5.3软件对菌株的耐药性进行分析。通过脉冲场凝胶电泳(PFGE)技术对菌株进行分子分型,通过同源性分析以确定菌株间的亲缘关系。[结果]20株宋内志贺菌对利福平和奈啶酸100%耐药,对四环素、氨苄青霉素、复方新诺明的耐药率≥85%。主要分为6种复合耐药型。19株宋内志贺菌被分成16个带型,聚类分析显示属于来源高度相似的克隆系。[结论]建立了本区的宋内志贺菌脉冲常凝胶电泳分析方法和初步带型数据库。宋内志贺菌存在多重耐药现象,抗生素型与基因型间无明确关系。[Objective]To study the genetic correlations and drug resistance of Shigella sonnei strains isolated from diarrhea in children in Jingan District of Shanghai.[Methods]Antimicrobial susceptibility tests were performed with 16 antibiotics according to the disk-diffusion method（Kirby-Bauer）.PFGE was performed according to unified Pulsenet USA protocol,so that to provide evidence for epidemiological source tracing and effective usage of antibiotics.[Results]20 strains of S.sonni were resistant t o Rifampicin（100%）and Nalidixic Acid（100%）.The resistant rate to tetracycline,ampicillin and compound sulfamethoxazole was over 85%.There were 6 patterns of multi-drug resistant.A total of 16 PFGE patterns were identified among the 19 isolates,and they were similar from one clone.[Conclusion]Primary PFGE patterns databank of Shigella sonnei isolated from Jingan District were established.There is no identified relationship between resistant phenotype and genotype.

关 键 词：宋内志贺菌 PFGE分型 药敏试验 多重耐药 

分 类 号：R378.25[医药卫生—病原生物学]