罗格列酮对胰岛素抵抗者的抵抗素基因表达水平的影响  

THE INFLUENCE OF ROSIGLITAZONE ON RESISTIN MRNA IN PATIENTS WITH INSULIN RESISTANCE

作　　者：张蔚然[1] 张蓓[2] 陈敏[3] 蒲丹[4] 杨瑛[5] 

机构地区：[1]四川省中西医结合医院(原四川省中医药研究院附属医院),成都610041 [2]成都市第二人民医院神经内科 [3]四川大学华西医院质量管理部 [4]四川大学华西医院呼吸内科 [5]四川大学华西体检中心

出　　处：《现代预防医学》2011年第22期4717-4719,共3页Modern Preventive Medicine

摘　　要：[目的]研究罗格列酮对胰岛素抵抗者的抵抗素基因表达水平的影响。[方法]将胰岛素抵抗患者的单个核细胞、单核源性巨噬细胞分别分为罗格列酮干预组(n=8)和对照组(n=8)。分别采用实时荧光定量PCR法、配对t检验检测、比较干预组和对照组细胞的抵抗素基因表达水平。[结果]罗格列酮干预组的单核源性巨噬细胞的抵抗素基因表达水平显著低于对照组(3.23±1.34vs0.94±1.22,t=3.565;P=0.009),两组单个核细胞的抵抗素基因表达水平无统计学差异。[结论]罗格列酮可以降低胰岛素抵抗者的单核源性巨噬细胞的抵抗素基因表达水平。罗格列酮可能是通过降低胰岛素抵抗患者的促炎症细胞因子水平而调控其炎症状况。[Objective]To explore the influence of rosiglitazone on resistin mRNA in patients with insulin resistance.[Methods]Mononuclear cells and monocyte-derived macrophages were cultured with or without rosiglitazone,respectively.Real time fluorescence quantitative PCR and paired-sample t test were applied to investigate and compare the mRNA of resistin between rosiglitazone and control group.[Results]For monocyte-derived macrophages,the mRNA level of resistin in rosiglitazone intervention group was significantly lower than that of control group（3.23±1.34 vs 0.94±1.22,t = 3.565;P = 0.009）.However,for mononuclear cells,the mRNA level of resistin in rosiglitazone group was not significantly different from that in control group.[Conclusion]For patients with insulin resistance,the mRNA level of resistin in monocyte-derived macrophages might be reduced by rosiglitazone.Rosiglitazone might regulate chronic inflammation by reducing proinflammatory factors in insulin resistance patients.

关 键 词：胰岛素抵抗 罗格列酮 抵抗素 

分 类 号：R977.15[医药卫生—药品]