当归多糖对小鼠化学性肝损伤抗氧化酶及一氧化氮含量的影响  

EFFECT OF ANGELICA SINENSIS POLYSACCHARIDE ON THE CONTENTS OF ANTI-OXIDATIVE ENZYMES AND NITRIC OXIDE IN MICE WITH CHEMICAL LIVER INJURED

作　　者：钟萍[1] 李萍[1] 张昕蕾[2] 

机构地区：[1]湖北郧阳医学院医疗保健中心,十堰442000 [2]北京市第二医院眼科

出　　处：《现代预防医学》2011年第22期4725-4727,共3页Modern Preventive Medicine

摘　　要：[目的]探讨当归多糖(angelica sinensis polysaccharide ASP)对CCL4肝损伤小鼠抗氧化酶、自由基和一氧化氮(nitric oxide,NO)的含量的影响。[方法]以4月龄昆明种小鼠为研究对象,分对照组、模型组和高、中、低当归多糖干预组,各组均给予常规饲料,自由饮水;干预组每天按体重用不同浓度的当归多糖灌胃,饲养7d,末次灌胃2h,正常对照组腹腔注射调和油溶液,其余各组腹腔注射0.15%CCl4调和油溶液,24h眼球取血,分离血清测ALT、AST。处死小鼠取出肝脏计算肝体指数,制备肝匀浆测定SOD、NOS活性和MDA及NO的含量。[结果]与模型组比较当归多糖能减轻化学性肝损伤小鼠炎性反应,降低MDA的含量(P﹤0.01)、增加SOD的活性(P﹤0.05～0.01),抑制NOS的活性和NO的含量(P﹤0.05～0.01)。[结论]当归多糖对CCL4肝损伤有保护作用。[Objective]To explore the effect of angelica sinensis polysaccharide（ASP）on the contents of anti-oxidative enzymes,free radicals and nitric oxide（NO）in the mice with CCl4 injured.[Methods]Four-month Kunming mice were divided into control group,model group,high-ASP-dosage,medium-ASP-dosage and low-ASP-dosage groups.All groups were given normal fodder and also free drinking.According to their body weight,intervening group were given different levels of ASP into stomachs for seven days.2 hours later,we fed the ASP at the last time.Control group were given mixed-edible-oil,the rest were given 0.15% CCl4 mixed-edible-oil,after 24h blood samples were prepared from eye balls and serum ALT and AST were determined.Afterwards,mice were killed and liver index were examined and acquaint liver were prepared to determined SOD and NOS activities and the levels of MDA and NO.[Results]ASP decreased hepatitis reaction and MDA contents（P﹤0.01）and increased SOD activities（P﹤0.05-0.01）and inhibit NOS activities and NO content（P﹤0.05-0.01）.[Conclusion]ASP can protect liver from damaging due to CCl4.

关 键 词：当归多糖 化学性肝损伤 抗氧化酶 一氧化氮 

分 类 号：R34[医药卫生—基础医学]