一个产氢产乙酸菌互营共培养体的培养基优化  

Composition optimization of medium for a syntrophic acetogen coculture

作　　者：李建政[1] 高晨晨[1,2] 张立国[1] 刘崇[1] 金羽[1] 张岩[1] 

机构地区：[1]哈尔滨工业大学市政环境工程学院城市水资源与水环境国家重点实验室,哈尔滨150090 [2]国家城市给水排水工程技术研究中心,天津300074

出　　处：《哈尔滨工业大学学报》2011年第10期40-44,共5页Journal of Harbin Institute of Technology

基　　金：国家自然科学基金面上项目(51178136);哈尔滨市科技创新人才专项基金项目(2009RFXXS004)

摘　　要：营养生态位位于产酸发酵菌和产甲烷菌之间的产氢产乙酸菌,其分离培养困难,种子资源匮乏,限制了基于强化产氢产乙酸功能作用的高效厌氧生物处理技术的开发.在前期获得对丙酸具有较强降解能力的产氢产乙酸菌互营共培养体7-m-2a的基础上,探讨了丙酸质量浓度、氮源、Ca2+、Fe2+、Mg2+和泛酸等对其生长代谢的影响.结果表明,培养基中适宜的丙酸钠和Fe2+、Mg2+、泛酸质量浓度分别为10 g/L和88、38、30 mg/L,最佳氮源是由质量浓度为0.33 g/L的酵母膏、胰蛋白胨和NH4Cl组成的复合氮源.在优化条件下38℃培养30 d,7-m-2a对丙酸的降解速率和乙酸产量分别可达998 mg/(L.d)和3 947 mg/L.Hydrogen -producing acetogens, between acidogenes and methanogens in trophic niche, is very difficult to be separated and only few of the pure cultures have been obtained. This has restricted the develop- ment of new anaerobic organic wastewater treatment process with high efficiency based on enhancing their met- abolic capability. A new syntrophic acetogen coculture named 7 - m - 2a which showed great capacity in de- grading propionic acid was introduced in this paper. To optimize the culture medium, the influences on 7 - m -2a＇ s growth and metabolism of propionic acid concentration, nitrogen sources, Fe^2＋ , Mg^2＋ , Ca^2＋ and pantothenic acid were investigated. The results indicated that the suitable concentration of propionic acid and Fe^2＋ , Mg^2＋ , pantothenic acid were 10 g/L and 88,38,30 rag/L, respectively. The optimum nitrogen source was the mixture of yeast extract, tryptone and NH4C1 with a concentration of 0. 33 g/L respectively. With the optimization culture, a specific degradation rate of propionic acid and acetic acid yield of 998 rag/（ L · d） and 3 947 rag/L, respectively, obtained after fermenting for 30 days at 38 %.

关 键 词：产氢产乙酸菌 互营共培养体 生长 代谢 培养基 

分 类 号：X382[环境科学与工程—环境工程] X172