胆绿素还原酶在大鼠纤维化肾脏中的分布及其与肾血管毁损的关系  

Expression of Biliverdin reductase in fibrotic kidney and its relationship with destruction of tubular interstitial vessels

作　　者：周逸婵[1] 裴广畅[1] 李彩霞[1] 张南[1] 白寿军[1] 韩敏[1] 曾锐[1] 徐钢[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院肾内科,湖北武汉430030

出　　处：《中国现代医学杂志》2011年第29期3595-3598,3603,共5页China Journal of Modern Medicine

基　　金：国家自然科学基金青年科学基金项目(No:30800525)

摘　　要：目的通过研究胆绿素还原酶(BVR)在肾脏组织中的表达部位及其与血管分布间的关系,为进一步研究其在肾脏纤维化中的作用提供依据。方法建立5/6肾纤维化(5/6Nx)模型,通过免疫组织化学、免疫荧光技术明确BVR在纤维化肾组织中的分布及其与血管分布的关系,Western blotting检测5/6Nx与Sham组BVR表达情况。结果 BVR主要在肾小管表达,肾小球表达较少;纤维化肾组织中血管分布较Sham组明显减少,血管损毁明显,而BVR的表达亦与血管分布相关,表现为血管分布少甚至没有的区域BVR的表达明显较血管分布密集的区域增强。结论 BVR在肾脏组织中的表达并不是均匀的,肾脏纤维化时血管损毁,BVR表达增强。[Objective] To study the relationship between BiliveMin reductase （BVR） and tubular intersti- tial vessels in the kidney of 5/6 nephrectomized rats. [Methods] Chronic renal failure was induced by 516 nephrectomy. Immunohistochemical SABC and Western blot methods were used to detect the expression of BVR at 12 weeks, immunofluorescence confocal Laser Scanning Microscopy was used to detect the expression of BVR and its relationship of the tubular interstitial vessels. [Results] When renal fibrosis occurred, tubular interstitial vessels of kidney were destroyed. The expression of BVR in the renal epithelium of 5/6 nephrec- tomized rats increased in contrast with sham group at 12 weeks, and there were significant correlations between BVR and tubular interstitial vessels： high density of tubular interstitial vessels with low expression of BVR, while low density of that with higher expression of BVR. [Conclusions] BVR expressed non-homogeneously in kidney, when renal fibrosis occurred, tubular interstitial vessels of kidney were destroyed, accompa- nied with higher expression of BVR.

关 键 词：胆绿素还原酶 肾纤维化 血管 

分 类 号：R692.5[医药卫生—泌尿科学] R-332[医药卫生—外科学]