平邑甜茶MhWRKY15基因cDNA克隆及其生物信息学分析  

Cloning of MhWRKY15 gene in Malus hupehensis and its bioinformatics analysis

作　　者：孙晓莉[1] 冉昆[1] 杨洪强[1] 李强[1] 姜倩倩[1] 

机构地区：[1]山东农业大学园艺科学与工程学院.作物生物学国家重点实验室.山东省果树生物学重点实验室,山东泰安271018

出　　处：《果树学报》2011年第6期949-952,共4页Journal of Fruit Science

基　　金：国家转基因生物新品种培育重大专项(2008ZX08009-3);高校博士点专项科研基金(20103702110003)

摘　　要：以平邑甜茶为材料,通过RT-PCR和RACE技术,获得平邑甜茶WRKY15基因的全长cDNA,命名为Mh-WRKY15(GenBank登陆号:GU576874)。生物信息学分析表明,该基因全长1 091 bp,包含810 bp的完整开放读码框,编码269个氨基酸,属于WRKY类转录因子的第II组,为WRKY15家族成员;其编码蛋白为可溶性蛋白,分子式为C1263H1941N365O425S12,相对分子量为29 423.2 ku,理论等电点为5.30;含有一个WRKY保守结构域,定位于细胞核,存在磷酸化、N糖基化和O糖基化位点,其二级结构主要以无规则卷曲为主。Full-length cDNA sequence of WRKY15 gene from roots of Malus hupehensis （Pamp.） Rehd. var. pinyiensis Jiang, which was tentatively designated as MhWRKYIS, with GenBank accession number GU576874, was acquired by RT-PCR and RACE with primers designed respectively based on the EST sequence. Bioinformatics analysis indicated that MhWRKY15 was 1 091 bp in length, containing an open reading frame of 810 bp and encoding 269 amino acids, and it belonged to group II of WRKY transcription factors and was one member of WRKY15 family. The results also showed that the protein encoded by MhWRKY15 was a soluble protein, with the molecular formula C_1263H_1941N_365O_425S_12, the relative molecular weight was 29 423.2 ku and the isoelectric point was 5.30. There was one conserved WRKY domain in the protein sequence. The protein might be located in nucleus, and there were many phosphorylation sites, N glycosylation sites and O glycosylation sites. The predicted secondary structure demonstrated that random coil was the most important structural conformation.

关 键 词：平邑甜茶 WRKY15 CDNA 生物信息学 

分 类 号：S661.19[农业科学—果树学]