甜樱桃B类MADS-box基因的分离与序列分析  

Isolation and sequence analysis of B-class MADS-box gene in sweet cherry

作　　者：林苗苗[1] 赵长竹[1] 顾红[1] 张慧琴[2] 陈锦永[1] 谢鸣[2] 方金豹[1] 

机构地区：[1]中国农业科学院郑州果树研究所.中国农业科学院果树生长发育与品质控制重点开放实验室,郑州450009 [2]浙江省农业科学院园艺研究所,杭州310021

出　　处：《果树学报》2011年第6期1082-1085,共4页Journal of Fruit Science

基　　金：十一五科技支撑资助项目(2006BAD07B06-10);浙江省科技计划面上项目(2009C32031);中国农业科学院果树生长发育与品质控制重点开放实验室资助项目(PP333KF20090101)

摘　　要：为研究MADS-box基因在甜樱桃花发育中的作用,以甜樱桃品种拉宾斯为试材,根据多种植物MADS-box基因保守序列设计引物,采用RT-PCR和RACE方法克隆基因cDNA全长,并用半定量PCR法研究基因在不同组织中的表达。克隆得到1个基因全长962 bp,命名为PaMADS2(GenBank登录号:HQ229606),包含1个633 bp的开放阅读框,编码210个氨基酸。序列比对和进化分析表明,PaMADS2与B类的PI基因家族亲缘关系最近。组织特异性分析表明PaMADS2在雄蕊和花瓣中表达。推断所克隆的PaMADS2基因为MADS-box B类家族成员。To study the role of MADS-box genes in floral development of sweet cherry, MADS-box gene was isolated from Prunus avium L. Lapins with RT-PCR and RACE approaches. Primers were designed according to the conservative region sequences of various plant MADS-box family genes, and semi-quan-titative PCR was used to analyze the expression of gene in different organs and tissues. The full length of cDNA is 962 bp, named PaMADS2 （the accession numbers in GenBank： HQ229606）, with an open reading frame （ORF） of 633 bp and encoding 210 amino acid residues. Sequence comparison and phylogenetic analysis indicated that PaMADS2 closely resembles the PI clade of class B. Tissue specific analysis showed that PaMADS2 expressed in petal and stamen. The results suggested that PaMADS2 belongs to the B-class MADS-box gene family.

关 键 词：甜樱桃 花器官 雄蕊 花瓣 MADS—box 

分 类 号：S662.5[农业科学—果树学]