藻粉中亚油酸GC测定的预处理方法研究  

Sample Pretreatment Methods for Linoleic Acid in Chlorella Powder by GC Analysis

作　　者：李金穗[1] 汪苹[1] 李奥搏[1] 

机构地区：[1]北京工商大学食品学院,北京100048

出　　处：《北京工商大学学报（自然科学版）》2011年第5期25-29,共5页Journal of Beijing Technology and Business University:Natural Science Edition

摘　　要：亚油酸作为一种新兴保健油脂受到越来越多的关注.研究了气相色谱法测定异养小球藻藻粉中亚油酸的预处理方法.通过单因素实验和正交试验,确定了优化工艺参数.结果表明:GC测定藻粉中亚油酸的优化预处理方法为酸催化甲酯化法,选用2mL体积比为1:1的正己烷/异丙醇溶液为提取剂,提取时间1h,加入3mL体积分数为2%的H2SO4-甲醇溶液甲酯化,1mL乙醚萃取.优化产率为3.00mg/g.Linoleic acid as a kind of novel functional oil,has received more and more attention.Sample pretreatment methods for linoleic acid in chlorella powder by GC analysis were studied in this paper.The optimal pretreatment conditions were established by single factor experiments and an orthogonal experiment.The best pretreatment method was acid-catalyzed methylation.The optimum parameters were listed as follows：2mL of N-hexane and isopropyl alcohol l（1：1）,Reaction time 1hour,3mL of 2% H2SO4-methanol solution for methylation,1mL of ether for methyl linoleate extraction.Under these conditions,the content of linoleic acid was 3.00mg/g.

关 键 词：异养小球藻 亚油酸 气相色谱 预处理 

分 类 号：TS224.4[轻工技术与工程—粮食、油脂及植物蛋白工程]