骨髓间充质干细胞复合聚乳酸-羟基乙酸支架材料移植修复大鼠脊髓损伤后膀胱功能的实验研究  

Bone Marrow Mesenchymal Stem Cells Combined with Polylactic Glycolic Acid Scaffold for Rat Treating Bladder Function after Spinal Cord Injury

作　　者：桂有富[1] 周兴[1] 符厚圣[1] 潘建刚[1] 

机构地区：[1]广州医学院第二附属医院泌尿外科,广东510260

出　　处：《国际泌尿系统杂志》2011年第6期717-721,共5页International Journal of Urology and Nephrology

基　　金：广东省自然科学基金项目（10151018201000019）

摘　　要：目的研究骨髓间充质于细胞（BoneMam）w Mesenchymal StemCells，BMSCs）及其分化生成的神经样细胞分别复合聚乳酸一羟基乙酸（Polylactie Glycolic Acid，PLGA）支架材料移植治疗大鼠脊髓损伤的效果。方法分别将单纯的PLGA、BMSCs—PLGA复合物以及神经样细胞一PLGA复合物移植入3组半横切的SD大鼠脊髓损伤模型中，对照组为直接缝合脊髓组。术后观察4组动物60d。结果BMSCs及其分化生成的神经样细胞能在PLGA上良好的黏附、增殖和生长，两者复合移植后能促进脊髓及膀胱排尿功能的恢复。结论BMSCs及神经样细胞复合PI．GA移植可作为组织工程治疗脊髓损伤后膀胱功能的一种方法。Objectives To study separately the effect of bone marrow mesenehymal stem cells （BMSCs） ~md diffcrentialed nenliated neural -like cells combined with polylactic glyeolic acid （PLGA） scaffolds,both composite which restore to SD rat, s hladder function aider spinal cord injury. Methods Bold scaffold, BMSCs - PLGA complex, and neural - like cells - PLGA complex were separately transplanted into SD rat models with the transection for right -pinal cord,the control group which is a direct suture half crosscut spinal eord group. And obsvered SD rat models ,thuring 60 days after operation. Results BMSCs and neural - like cells can be exactly adhere to, proliferate and grow on the PLGA;BMSCs and neural - like cells combined with PLGA,which can restore to spinal cord and promote the recovery of bladder function of SD rat models. Conclusions BMSCs and differentiated neural - like cells ,combined with PLGA scaffolds, which can be used as a method of tissue engineering, to treat bladder function caused by spinal cord injury.

关 键 词：脊髓损伤 膀胱 干细胞 骨髓细胞 大鼠 

分 类 号：R694.5[医药卫生—泌尿科学] R651.2[医药卫生—外科学]