肾损伤分子-1、尿N-乙酰-β-D-氨基葡萄糖苷酶及尿微量白蛋白在重症感染中合并急性肾损伤的临床观察  

Clinical observation ot kidney injury molecule - 1 ） urine N - acetyl - beta - D - glucosaminidase and urine microalbumin in severe infection with acute kidney injury

作　　者：岳华 刘红 刘颖 赵志刚 

机构地区：[1]乌鲁木齐维吾尔自治区人民医院肾脏病研究室,新疆830001

出　　处：《国际泌尿系统杂志》2011年第6期730-732,共3页International Journal of Urology and Nephrology

基　　金：新疆维吾尔自治区人民医院课题资助（项目编号：20100118）

摘　　要：目的探讨尿肾损伤分子-l（Kjm-1）、尿N-乙酰-β-D-氨基葡萄糖苷酶（N—ace-cyl-β-D—glucosaminidase，NAG）及尿微量白蛋白（mALB）在重症感染中合并急性肾损伤的敏感性及临床价值。方法回顾分析60例在本院ICU住院的重症感染合并急性肾损伤（AKI）患者的尿Kim-l、尿NAG及尿mALB的变化情况。健康体检者20例为对照组。尿Kim-l、mALB测定采用酶联免疫法（ELISA）检测，尿NAG测定采用对硝基苯酚（PNP）比色法检测。结果AKI组患者尿液中的Kim-1、NAG、mALB的测定浓度明显高于对照组，差异具有统计学意义（P〈0．001），通过ROC曲线、诊断试验结果显示：尿Kim-1曲线下面积为0．955，95％可信区间为0．910-1．000，较尿NAG、mALB更具有敏感性（P〈0．001），两组问患者年龄及男女比例差异均无统计学意义（P〉0．05）。结论尿Kim-1对重症感染中合并急性肾损伤有诊断意义，检测尿Kim—l的浓度有助于急性肾损伤的早期诊断，对预防急性肾损伤的发生、发展具有重要的临床价值，Objectives To explore the sensitivity and clinical value of urine kidney injury molecule - 1 （ Kim - 1 ） ,urine N - acetyl - beta - D - glucosaminidase（NAG） and urine microalbumin（mALB） in patients of severe infection with acute kidney injury. Methods To retrospectively analysis the changes of urine Kim - l, urine NAG and urine mALB in 60 cases of severe infection with acute kidney injury who hospitalized in ICU. 20 healthy subjects as control group. The urine Kim - 1 and urine mALB were respectively determined by enzyme linked immunosorbent assay （ ELISA ）. Urine NAG was measured by p - nitrophenol （PNP） colorimetric assay. Results Urine Kim - 1, urine NAG and urine mALBwere significantly higher in AKI group, compared with control group, there were statistically signifieanees between AKI group and control group （ p 〈 0. O01 ）. Through the ROC curve and diagnostic test ,the results showed that, the area under the curve of urine Kim - 1 was 0. 756, the 95% confidence interval was 0.910- 1.O00,urine Kimlwas more sensitive than urine NAG and urine mALB （ p 〈0.001 ） , No statistically significant difference was found in age,gender proportion between AKI group and control group（ p 〉 0.05 ）. Conclusions Urine Kiln - 1 had the diagnosis significance for patients of severe infection with acute kidney injury , detecting Urine Kim - 1 was helpful for the early diagnosis of acute kidney injury, it had important clinical value on the prevention in pathogenesis and development of acute kidney injury.

关 键 词：肾疾病 感染 乙酰氨基葡糖苷酶 白蛋白类 

分 类 号：R692[医药卫生—泌尿科学]