盐酸氮芥抑制人肝癌HepG2细胞生长的机制探讨  

An Investigation of the Probable Mechanism of Nitrogen Mustard to Suppress Liver Cancer HepG2 Cells Growth

作　　者：奉水东[1] 凌宏艳[2] 

机构地区：[1]南华大学社会医学与卫生事业管理学教研室,湖南衡阳421001 [2]南华大学生理学教研室

出　　处：《中南医学科学杂志》2011年第5期497-500,共4页Medical Science Journal of Central South China

基　　金：湖南省自然科学基金(10JJ6046);衡阳市科技局课题项目(2008KS032)

摘　　要：目的探讨盐酸氮芥抑制人肝癌HepG2细胞生长的可能机制。方法以盐酸氮芥处理人肝癌HepG2细胞,运用DNA片断化分析、流式细胞术检测肝癌HepG2细胞的凋亡和细胞周期的改变。结果 DNA片断化分析显示没有出现典型"梯形"条带,流式细胞术显示各浓度组细胞凋亡率之间差异无显著性(P>0.05),但有明显的细胞周期阻滞(S期和G2期)。结论盐酸氮芥对人肝癌HepG2细胞的生长抑制主要通过细胞周期的阻滞发挥作用。Objective To investigate the probable mechanism of nitrogen mustard to suppress human liver tumor HepG2 cells growth. Methods The apoptctic cells and the cell cycle were detected by DNA fragmentation analysis and flow cytometry. Results The ladder-shaped strands weren＇t observed from DNA fragmentation analysis ;There was no significant difference in apoptotic rate among various concentration groups of nitrogen mustard by flow cytometry（ P 〉 0.05 ）, but cell cycle arrest was distinctly observed at S phase and G2 phase. Conclusions Nitrogen mustard suppressed liver cancer HepG2 cells growth mostly by cell cycle arrest.

关 键 词：盐酸氮芥 凋亡 细胞周期 肝癌HEPG2细胞 

分 类 号：R979.1[医药卫生—药品]