NO-1886对高脂/高糖/高胆固醇饲养的小型猪组织中CRP和IL-6蛋白表达的影响  

Effects of NO-1886 on the Protein Expressions of CRP and IL-6 in High Fat/High Sucrose/High Cholesterol Diet Fed Miniature Pigs

作　　者：蔡曼波[1] 李茂凤 李建军[3] 何平平[1] 胡丽[1] 廖如意[1] 周钰娟[1] 尹卫东[4] 

机构地区：[1]南华大学护理学院,湖南衡阳421001 [2]衡阳市第三人民医院 [3]南华大学第二附属医院肾移植科 [4]南华大学心血管病研究所

出　　处：《中南医学科学杂志》2011年第5期507-510,共4页Medical Science Journal of Central South China

摘　　要：目的探讨脂蛋白酯酶活化剂NO-1886对高糖/高脂/高胆固醇饲养的小型猪组织中CRP及IL-6蛋白表达的影响。方法 15只雄性巴马小型猪,按体重随机分为3组:正常组:喂基础猪饲料;三高组:喂高脂/高蔗糖/高胆固醇饲料(含10%猪油、37%蔗糖和2%胆固醇);加药组:在高脂/高蔗糖/高胆固醇饲料里加1.0%NO-1886。5个月后处死动物,用油红O染色检测脂质在小型猪肝脏组织的蓄积情况,Western blot技术检测炎症因子CRP和IL-6在肝脏和脂肪等组织中的表达。结果正常组和加药组肝脏结构正常,未见明显脂滴;而三高组肝脏组织内充满了大量大小不等的红染脂滴。与正常组比较,高脂/高糖/高胆固醇饮食能诱导巴马小型猪肝脏和脂肪等组织中CRP和IL-6的蛋白表达增加,而在高脂/高糖/高胆固醇饲料中添加1.0%NO-1886后,NO-1886能降低肝脏和脂肪等组织中CRP和IL-6的蛋白表达。结论 NO-1886能明显抑制脂质在肝脏组织中的蓄积,降低高脂/高糖/高胆固醇饲养的小型猪组织中CRP及IL-6的蛋白表达。Objective To investigate the role of lipoprotein lipase （LPL） activator NO-1886 on the protein expressions of C reactive protein（CRP） and IL-6 in high-fat/high-sucrose/high-cholesterol diet fed miniature pigs. Methods Male ,fifteen Bama-miniature pigs were randomized into three groups ： control group, high-fat/high-sucrose/high-cholesterol （control diet supplemented with 10% lard and 37% sucrose and 2% cholesterol. ） group and NO-1886 treated group. The pigs were sacrificed at the end of month 5. The degree of lipid deposition in liver tissue was examined by oil red O stain. The protein levels of CRP and IL-6 in liver and fat tissues were measured by western blot. Results The high-fat/high-su- crose/high-cholesterol diet induced large amount of lipid droplets deposition in liver tissue compared with normal diet group and NO-1886 treated group, increased the protein levels of CRP and IL-6 in liver and fat tissues compared with the normal diet group. While NO-1886 ameliorated visceral fat deposition and reduced the protein expression of CRP and IL-6 in liver and fat tissues. Conclusions NO-1886 may significantly inhibit eetopic visceral lipid deposition,decrease the protein expression of CRP and IL-6 in tissues of high-fat/high-sucrose/high- cholesterol diet fed miniature pigs.

关 键 词：NO-1886 脂蛋白酯酶 CRP IL-6 动脉粥样硬化 

分 类 号：R541.4[医药卫生—心血管疾病]