液-液萃取反相离子对色谱法测定口腔崩解片剂中溴吡斯的明的血浆浓度  

Determination of Plasma Concentration of Pyridostigmine Bromide in Oral Disintegrating Tablets by Liquid-liquid Extraction Reversed Phase Ion Pair Chromatography

作　　者：张梨[1] 谭群友[2] 柳珊[1] 罗文[1] 张景勍[1] 

机构地区：[1]重庆医科大学药物高校工程研究中心和生物化学与分子药理学重点实验室,重庆市渝中区医学院路1号400016 [2]第三军医大学大坪医院野战外科研究所胸外科中心,重庆市渝中区长江支路10号400042

出　　处：《光谱实验室》2011年第6期2753-2758,共6页Chinese Journal of Spectroscopy Laboratory

基　　金：教育部博士点基金资助项目(20095503120008);重庆市教育委员会首批高等学校优秀人才资助(KJ090308)

摘　　要：建立了反相离子对色谱法测定口腔崩解片剂中溴吡斯的明血浆浓度的方法。血浆样品经液-液萃取法处理,采用反相离子对色谱法分离,以溴新斯的明作为内标定量。实验结果显示溴吡斯的明和内标物洗脱时间分别为5.63min和9.76min,与血浆中内源性杂质良好分离。溴吡斯的明线性范围为0.02—2.00mg.L-1(n=7,r=0.9994);样品绝对回收率为82.0%—89.2%,相对回收率为98.0%—100.0%,日内和日间RSD分别为2.0%—4.1%和3.1%—6.7%;当S/N=3时,溴吡斯的明检出浓度可达0.005mg.L-1。本方法准确、灵敏,可满足血药浓度检测和研究药代动力学行为的需要。The method for the determination of the plasma concentration of pyridostigmine bromide in oral disintegrating tablets was established by liquid-liquid extraction reversed phase ion pair chromatography.The plasma samples were treated by liquid-liquid extraction method by reversed phase ion pair separation method with pyridostigmine bromide as internal standard quantitative.The elutiontime of pyridostigmine bromide and internal standard substance were 5.63min and 9.76min,respectively,and that good separated with endogenous impurities in plasma.The linear range of pyridostigmine bromide was 0.02—2.00mg·L-1（n=7,r=0.9994）,and absolute recoveries of samples were from 82.0%—89.2%,while relative recoveries were between 98.0% and 100.0%,and RSDs of inter-day and intra-day were 2.0%—4.1% and 3.1%—6.7%,respectively.When ratio of S and N was 3,the detection concentration of pyridostigmine bromide could be up to 0.005mg·L-1.The method is accurate and sensitive,and that can meet the need for the detection of plasma concentration and research on pharmacokinetic behavior.

关 键 词：溴吡斯的明 液-液萃取 离子对色谱法 口腔崩解片 

分 类 号：O657.75[理学—分析化学]