双波长分光光度法同时测定载硫酸长春新碱/姜黄素聚合物纳米粒中两种药物的含量  

Simultaneous Determination of the Two Drugs in Polymeric Nanoparticles Loaded with Vincristine Sulfate and Curcumin by Dual-Wavelength Spectrophotometry

作　　者：王海鸥[1,2] 袁佩[2] 滕永真[2] 贾运涛[3] 张良珂[1,2] 

机构地区：[1]重庆医科大学药学院重庆市生物化学与分子药理学重点实验室,重庆市400016 [2]重庆医科大学生命科学院,重庆市400016 [3]重庆医科大学附属儿童医院药剂科,重庆市400010

出　　处：《光谱实验室》2011年第6期2820-2824,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：国家自然科学基金项目(30770565);重庆市科委自然科学基金资助项目(CSTC;2008BB5397);重庆市渝中区科技计划项目资助(20100203);重庆市卫生局课题资助(2010-2-202)

摘　　要：以聚乙二醇单甲醚-聚乳酸-乙醇酸共聚物(m PEG-PLGA)为载体材料,制备同时载带硫酸长春新碱(VCR)和与之具有协同抗肿瘤作用的姜黄素(Cur)的m PEG-PLGA纳米粒(VCR/Cur-m PEG-PLGA-NP)。建立了以等吸收双波长紫外分光光度法同时测定纳米粒中VCR和Cur含量的方法,研究显示,VCR测定波长296.5nm,等吸收波长462nm;Cur测定波长414nm;VCR在4.0—36.0μg.mL-1范围内,浓度C与差值吸光度ΔA呈良好线性相关;Cur在0.5—8.0μg.mL-1范围内,浓度C与吸光度A具有良好的线性关系。该方法简便迅速,结果准确可靠,适用于VCR/Cur-m PEG-PLGA-NP中药物含量的同时测定。mPEG-PLGA nanoparticles simultaneously loaded with vincristine sulfate（VCR） and curcumin（Cur） were prepared with polyethylene glycol momoethyl ether-polylactic acid-glycollic acid copolymer（mPEG-PLGA） as carrier material.Dual-wavelength ultraviolet spectrophotometry was employed for measuring the content of the dual agent（VCR and Cur） in VCR/Cur-mPEG-PLGA-NP.The results showed that 296.5nm and 462nm were the maximum absorption wavelength and isosbestic wavelength of VCR respectively,and 414nm was the maximum absorption wavelength of Cur.Good linear correlations between the content of drug and absorbance were found in the range of 4.0—36.0μg·mL-1 for VCR and 0.5—8.0μg·mL-1 for Cur.In conclusion,a simple,accurate and sensitive method was established to simultaneously determine the content of VCR/Cur-mPEG-PLGA-NP.

关 键 词：双波长 紫外分光光度法 姜黄素 硫酸长春新碱 

分 类 号：O657.32[理学—分析化学]