黄绿蜜环菌菌丝体水提物多糖含量测定  

Determination of Polysaccharide in Armillarialuteo-rivens Mycelium Extract

作　　者：党军[1,2] 王瑛[1,2] 陶燕铎[1] 邵赟[1] 梅丽娟[1] 王启兰[1] 

机构地区：[1]中国科学院西北高原生物研究所,西宁市西关大街59号810008 [2]中国科学院研究生院,北京市100049

出　　处：《光谱实验室》2011年第6期2836-2840,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：国家科技支撑计划项目(2007BAI45B00)

摘　　要：采用苯酚-硫酸法测定了黄绿蜜环菌菌丝体水提物中多糖的含量。测定波长492nm,葡萄糖质量浓度与吸光度的回归方程为A=0.051C-0.1150,R2=1.0000,其平均回收率为102.22%,相对标准偏差为3.54%(n=6)。测得黄绿蜜环菌菌丝体水提物多糖含量为31.21%。The content of polysaccharides in Armillarialuteo-rivens mycelium extract was measured by phenol-sulfuric acid method.Wavelength for measurement was 492nm.The linear relationship of absorbance of glucose with its concentration was good with regression equation A=0.051C-0.1150（R2=1.0000）,average recovery of glucose was 102.22% with RSD of 3.54%（n=6）.The contents of polysaccharides in Armillarialuteo-rivens mycelium extract was 31.21%.

关 键 词：黄绿蜜环菌菌丝体 多糖 苯酚-硫酸法 

分 类 号：O657.32[理学—分析化学]