PRRSV浙江分离株Nsp2基因序列分析  

Sequence Analysis of Nsp2 Genes of PRRSV Isolates from Zhejiang

作　　者：张涵淞[1] 扈鸿霞[1] 赵灵燕[2] 周彩琴[2] 李肖梁[1] 方维焕[1] 徐辉[2] 

机构地区：[1]浙江大学浙江省动物预防医学重点实验室,浙江杭州310029 [2]浙江省动物疫病预防控制中心,浙江杭州310020

出　　处：《动物医学进展》2011年第11期20-25,共6页Progress In Veterinary Medicine

基　　金：浙江省重大科技专项农业资助项目(2007C12045)

摘　　要：猪繁殖与呼吸综合征(PRRS)临床上主要表现为流产、早产、死胎、仔猪成活率下降和育成猪呼吸系统病变等。本研究用RT-PCR对2008年-2009年采集于浙江省部分发病猪场的41株疑似猪繁殖与呼吸综合征病猪的脾、肺、淋巴结混合组织样本进行PRRSV病毒检测和Nsp2基因序列分析。结果显示,41株PRRSV流行毒株中20份为NSP2缺失毒株,与国内分离株JXA1、HuN、HUN4等具有相同的缺失特征。41个毒株之间同源性为89.2%～100.0%,其中20个缺失株之间核苷酸同源性为94.2%～99.8%;21个非缺失株之间核苷酸同源性为99.2%～100.0%。The Nsp2-specific RT-PCR method was used to detect partial gene deletion of Nsp2 of porcine reproductive and respiratory syndrome virus（PRRSV） from 41 PRRSV-positive mixed tissue samples,including spleen,lung and lymph nodes of diseased pigs from Zhejiang province from 2008 to 2009.The PCR products were cloned and sequenced.The results showed that the 90-base deletion in Nsp2 was found in 20 out of the 41 PRRSV-positive samples.The deletion site was the same as the other PRRSVs published in the literature.There was nucleotide identity of 89.2%-100.0% within the 41 isolates.The nucleotide homology of 20 PRRSV with discontinuous Nsp2 deletion was 94.2%-99.8% while that of the other 21 PRRSV without partial Nsp2 deletion was 99.2%-100.0%.

关 键 词：猪繁殖与呼吸综合征病毒 NSP2 序列分析 

分 类 号：S852.659.6[农业科学—基础兽医学]