羔羊超数排卵与胚胎体外生产技术研究进展  

Advance in Goat and Sheep Juvenile in Vitro Embryo Transfer

作　　者：李运生[1,2] 曹鸿国[1,2] 刘亚[1,2] 韩春杨[1] 陶勇[1,2] 张运海[1,2] 

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230036 [2]安徽地方畜禽遗传资源保护与生物育种省级实验室,安徽合肥230036

出　　处：《动物医学进展》2011年第11期99-104,共6页Progress In Veterinary Medicine

基　　金：转基因生物新品种培育重大专项基金(2008ZX08008-005;2009ZX08006-007B)

摘　　要：自从幼畜体外胚胎移植(JIVET)技术问世以来,因其在优秀种畜快繁、育种世代间隔缩短、遗传资源保护等方面具有广阔的应用前景,引起了很多科研机构的关注。为提高JIVET的生产效率,研究人员对该技术的多个环节进行了探索,但总体效率还不是很高。论文就羔羊超数排卵与胚胎体外生产技术实施中的关键环节,即羔羊超排供体的年龄、超排季节生殖激素的使用、羔羊超排反应、卵母细胞发育效率、卵母细胞的基因表达、体外胚胎的冷冻耐受性和卵母细胞及受精卵培养体系的优化等影响因素进行总结与分析,提出羔羊超排存在的问题并展望其发展前景,为这一技术能在更多家畜繁殖领域的应用和商业化生产提供参考资料。Juvenile in vitro embryo transfer（JIVET） is an important animal reproductive technology and always concerned by many academies or institutes for its broad potential value in reproducing quickly domestic animals with good property,reducing the generation duration of breeding and reserving genetic resources and so on.However,efficiency of JIVET was not high,so many researchers were dedicated to improve the efficiency of JIVET.The current status of JIVET and a number of factors affecting its efficiency including selection of the donor,hormone stimulation prior to oocyte collection,gene expression,developmental capabilities,cryopreservation tolerance,in vitro culture system of prepubertal oocytes and IVF embryos were described.Problems and future fields of JIVET were summarized as to provide theoretical references for commercial production and applications in other domestic animals.

关 键 词：羔羊 超数排卵 幼畜体外胚胎移植 胚胎 卵母细胞 

分 类 号：S821.36[农业科学—畜牧学]