氧化低密度脂蛋白诱导心肌细胞LOX-1表达的研究  

Expression of lectin like oxidized low density lipoprotein receptor-1 in cardiomyocytes induced by ox-LDL

作　　者：胡波[1,2] 李德才[1,2] 

机构地区：[1]川北医学院附属第二医院 [2]四川绵阳四0四医院心内科,四川绵阳621000

出　　处：《西部医学》2011年第11期2074-2076,共3页Medical Journal of West China

摘　　要：目的探讨氧化低密度脂蛋白(ox-LDL)对心肌细胞血凝素样氧化低密度脂蛋白受体1(LOX-1)表达的影响。方法原代培养乳鼠心肌细胞,分别用不同浓度的ox-LDL(0,10,20,50,100μg/ml)与心肌细胞作用不同时间(0,6,12,24,36h)。用RT-PCR测定LOX-1mRNA表达水平,Western blot测定LOX-1蛋白表达水平。结果不同浓度的ox-LDL均可诱导心肌细胞LOX-1mRNA和蛋白表达,与对照组比较有显著性差异(P<0.05),在10-50μg/ml的剂量范围内存在明显的剂量-效应关系,各组间比较有显著性差异(P<0.05)。随着ox-LDL与心肌细胞作用时间的延长,心肌细胞LOX-1mRNA和蛋白表达增加,与对照组比较差异具有显著性(P<0.05),在6～24h内呈明显的时间-效应关系,各组间比较有显著性差异(P<0.05)。结论 ox-LDL可诱导心肌细胞LOX-1表达,并在一定范围内随ox-LDL浓度增加和作用时间延长而表达增强。Objective To investigate the expression of lectin like oxidized low density lipoprotein receptor-1（LOX-1） of rat cardiomyocytes induced by oxidized low density lipoprotein（ox-LDL）.Methods Primary cardiomyocytes were isolated from Sprague-Dawley neonatal（1 to 2 days old） rats ventricles.Neonatal rat ventricular myocytes were incubated with ox-LDL（0,10,20,50 and 100μg/ml） at different time（0,6,12,24 and 36h）.LOX-1 mRNA and protein expression were determined by RT-PCR and Western blot analysis,respectively.Results Ox-LDL induced the expression LOX-1（mRNA and protein） in a concentration-dependent manner compared with control group（P〈0.05）.Incubation of cardiomyocytes with 50μg/ml ox-LDL at different time also increased the expression of LOX-1（mRNA and protein）.Conclusion Ox-LDL significantly increases LOX-1 expression in concentration-dependent and time-dependent manners.

关 键 词：氧化低密度脂蛋白 心肌细胞 血凝素样氧化低密度脂蛋白受体1 

分 类 号：R972.9[医药卫生—药品]