白细胞介素10-819C/T、-1082G/A基因多态性与脑梗死的关系  

The correlation between gene polymorphism of IL-10-819C/T and-1082G/A and cerebral infarction

作　　者：金磊[1] 倪培华[2] 吴洁敏[2] 傅毅[3] 葛海良[1] 

机构地区：[1]上海交通大学医学院免疫教研室,上海200025 [2]上海交通大学附属瑞金医院检验系,上海200025 [3]上海交通大学附属瑞金医院神经内科,上海200025

出　　处：《检验医学》2011年第11期717-721,共5页Laboratory Medicine

摘　　要：目的探讨白细胞介素10(IL-10)基因多态性与脑梗死(CI)的关系。方法采用突变错配扩增技术,结合血液生化和血压等临床资料,对189例急性CI患者(CI组)和92例非急性CI者(对照组)的IL-10启动子1082G/A和819C/T基因的单核苷酸多态性(SNP)位点与CI的关系进行研究。结果 IL-10启动子1082G/A和819C/T的CI组的基因型和等位基因频率分布与对照组相比差异均无统计学意义(P>0.05);CI组的IL-10启动子1082AA型的收缩压明显高于(AG+GG)型(P<0.05),且AA型的收缩压、舒张压、血糖均明显高于对照组(P<0.05),(AG+GG)型的高密度脂蛋白胆固醇(HDL-C)明显低于对照组(P<0.05);CI组的IL-10启动子819CC型的收缩压、舒张压均明显高于TT型和CT型(P<0.05),且TT型的收缩压、舒张压、血糖均明显高于对照组(P<0.05),CT型的HDL-C明显低于对照组(P<0.05),CC型的收缩压、舒张压明显高于对照组(P<0.05)。结论 IL-10基因启动子1082G/A和819C/T的多态性与CI发生无明显相关,但与CI的发展和预后有一定关联。Objective To investigate the correlation of interleukin-10 （IL-10） gene polymorphism and cerebral infarction （CI）. Methods A total of 189 patients with acute CI （ CI group） and 92 controls with non-acute CI （ control group） were detected by mismatch amplification mutation assay （MAMA）. Combined with clinical data and information, the single nucleotide polymorphism （SNP） loci of IL-10 -1082G/A and -819C/T gene, and the correlation with CI was analyzed. Results There was no statistical difference in the distribution of genotypes and allele frequencies of IL-10 -1082C/A and -819 C/T between the CI group and control group （P 〉 0.05）. In the CI group, systolic blood pressure was significantly higher in 1082 AA type than in （AG ＋ GC）type （P 〈 0.05 ）. Systolic blood pressure, diastolic blood pressure and glucose of the CI group in AA type were significantly higher than those of the control group （ P 〈 0.05 ）, and high-density lipoprotein cholesterol （HDL-C） of the CI group in （AG ＋ CC） type was significantly lower than that of the control group （P 〈 0.05 ）. Systolic blood pressure and diastolic blood pressure were significantly higher in 819 CC type than Tr and CT types of the Cl group （ P 〈 0.05 ） , and systolic blood pressure , diastolic blood pressure and glucose of the CI group in Tr type were significantly higher than those of the control group （ P 〈 0.05 ） , and HDL-C of the CI group in CT type was significantly lower than that of the control group （ P 〈 0.05 ）. Systolic blood pressure and diastolic blood pressure of the CI group in CC type were significantly higher than those of the control group （ P 〈 0.05 ）. Conclusions IL-10 -1082C/A and -819C/T polymorpbisms are unlikely to be correlated with the prevalence of CI,but have a possible correlation with the incidence and prognosis of CI.

关 键 词：白细胞介素10 基因多态性 脑梗死 

分 类 号：Q503[生物学—生物化学]