半固相生化反应试剂板的研制  

Development of semi-solid state reagent plates used for biochemical detection

作　　者：高德禄[1] 张世俊[1] 牛刚[2] 张金树[1] 

机构地区：[1]解放军第305医院检验科,北京100017 [2]北京牛牛基因有限公司,北京100034

出　　处：《中国医学装备》2011年第11期5-7,共3页China Medical Equipment

基　　金：全军十一五科研攻关基金课题(08G056)

摘　　要：目的:研制适合野外环境下进行生化项目检测的试剂板。方法:采用半固相化学反应技术,将所需试剂固定于载体板上,微量样品可通过低速离心准确与试剂发生反应并产生颜色,通过分光比色计算出检测项目的含量。结果:项目包括钾、钠、血糖、尿素、肌酐、转氨酶。收集50份标本,在大型生化自动仪上进行相同项目检测,相关性良好,r>0.95。结论:此方法性能可达到常规化学法水平,且快捷灵活,尤其适用于评估基层单位军民的健康情况及灾难和战伤时的救治需要。Objective：To develop semi-solid reagents plates used for a portable device adapting to the outer environment. Methods：Semi-solid chemical reaction was applied and the reagents required for the testing items were fixed on the carrier plate when the tiny blood sample could react with the reagent chamber and produce color after the accurate low-speed centrifugation, and the color of the final test represents the content of the corresponding items. Results：The semi-solid biochemical reagent plate mainly included potassium, sodium, glucose, urea, creatinine and transaminases. Fifty samples were collected, and the results got from the auto analyzer and this portable device were compared , showing relatively good correlation, r0.95. Conclusion：The performance of detection could match the level of conventional biochemical method, and it has unique advantage, such as flexible and cost-effective mobility, especially helpful to evaluate the health of the common people and soldiers in the elementary army units and satisfy the urgent need of disaster and war wounds treatment.

关 键 词：半固相化学反应 即时检验 试剂板 

分 类 号：R446[医药卫生—诊断学]