黑脊倒刺鲃scp3基因的克隆及其表达  

Molecular Cloning and Expression of scp3 Gene in Spinibarbus caldwelli

作　　者：苏敏[1] 吕博彦[1] 唐良华[1] 詹冰津[1] 

机构地区：[1]福建师范大学生命科学学院,福建福州350108

出　　处：《福建师范大学学报（自然科学版）》2011年第6期71-76,共6页Journal of Fujian Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(81072616);福建省自然科学基金资助项目(2011J01149);福建省教育厅资助项目(JA09063)

摘　　要：为了从分子水平上研究鱼类生殖细胞发育的机制,首次从重要的经济养殖鱼类黑脊倒刺鲃中克隆出了生殖细胞减数分裂Ⅰ相关基因scp3的部分同源序列scscp3:该序列长477bp,编码159个氨基酸.经比对发现,其编码的蛋白序列与斑马鱼scp3蛋白等具有高度的同源性.不同组织RT-PCR结果显示:scscp3基因仅在生殖细胞中表达,可将其作为生殖细胞的标记基因,用于鱼类生殖发育的研究.RNA原位杂交结果表明:在精子发生中,scscp3基因均是在精原细胞和初级精母细胞中表达,在后期的精子细胞中未发现表达.而在卵子发生中,scscp3基因在卵原细胞和卵母细胞的各个时相中均有表达,并且在卵原细胞和前期的卵母细胞中表达强烈,在后期时相的卵母细胞中阳性信号逐渐减弱,且向细胞边缘聚集.scp3 homolog（germ cell meiosisⅠrelated gene） from Spinibarbus caldwelli was successfully cloned in order to interpret the development mechanism of fish germ cells at molecular level.Spinibarbus caldwelli scp3（scscp3）homolog was partial open reading frame of 477 bp,encoding 159 amino acids.Also,Spinibarbus caldwelli scp3 showed highly homologous to that of Danio rerio et al.Semi-quantitative RT-PCR showed that scscp3 were specifically expressed in the gonad tissue of adult Spinibarbus caldwelli.So,it could be used as a useful marker for development research of fish germ cell.In situ hybridization analysis result was as follows： scscp3 was transcripted both in spermatogonia and spermatocytes,but not in spermatoblasts and sperms.During oogenesis,scscp3 was transcripted in all the time phase from oogonia to oocytes.The earlier phase of oogenesis,the more scscp3 mRNA molecules were transcripted.The positive signals of scscp3 mRNA gradually weakened in the late phase of oocytes and finally these signals gathered round the cell edge.

关 键 词：黑脊倒刺鲃 scp3 原位杂交 生殖细胞 

分 类 号：Q785[生物学—分子生物学]